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2019 Vol.32, Issue 3 Preview Page

Research Article

Optimized Shoot Induction and Histological Study of in vitro Cultured Korean Soybean Cultivars
Kantayos Vipada1
,
Chang-Hyu Bae2*
1Post-doc, Department of Well-being Resources, Sunchon National University
2Professor, Department of Well-being Resources, Sunchon National University
* Corresponding Author
30 June 2019. pp. 237-243
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Abstract

Soybean is the one of recalcitrant legume species for shoot induction. Shoot regeneration via direct organogenesis was investigated in five soybean cultivars, ‘Dawon’, ‘Pungsan’, ‘Daewon’, ‘Taekwang’ and ‘Chongdoo 1’ by using cotyledonary node explants. Out of 5 soybean cultivars, an efficient shoot regeneration condition was developed in the two soybean cultivars, ‘Dawon’ and ‘Pungsan’. When various kinds of plant growth regulators with different concentration were estimated, the optimum medium condition for shoot induction in both soybean cultivars was MS + B5 vitamin supplemented with BA at concentration 2 ㎎/L. In addition, shoot formation efficiency was increased with 97.09% and 93.88% by the pretreatment of BA onto the explants before in vitro culture in both cultivars. Shoot induction in ‘Dawon’ cultivar was originated from epidermal tissue and sub-epidermal layers when histological changes were investigated under shoot regeneration after culturing cotyledonary node segments on shoot induction medium for 0 to 21 days. Especially, cell dedifferentiation was observed from parenchyma cells to meristematic cell in 3-day cultured segments.

Keywords
Cotyledonary node
Histological study
in vitro culture
Shoot induction
Soybean cultivar
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :3
  • Pages :237-243
  • Received Date : 2019-06-06
  • Revised Date : 2019-06-13
  • Accepted Date : 2019-06-13
  • DOI :https://doi.org/10.7732/kjpr.2019.32.3.237
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