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2018 Vol.31, Issue 6 Preview Page

Research Article

A Study on Antimicrobial Activity of Lysimachia clethroides Duby Root Extracts against Methicillin-resistant Staphylococcus aureus
Dae Ki Joung1
,
Dong Yeul Kwon2
,
Dong Won Shin3*
1Graduate Student, Department of Development in Oriental Medicine Resources, Sunchon National University
2Professor, Department of Oriental Pharmacy, Wonkwang University
3Professor, Department of Development in Oriental Medicine Resources, Sunchon National University
*Corresponding Author
31 December 2018. pp. 719-724
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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for a number of infections in humans that are difficult to treat, and as a result, is a substantial contributor to morbidity and mortality. In the present study, in search of natural products capable of inhibiting this multidrug-resistant bacterium, we investigated the antimicrobial activity of Lysimachia clethroides Duby root. The antibacterial activities of EtOH extract of Lysimachia clethroides Duby root and its n-hexane, EtOAc, n-BuOH and water fractions were evaluated against 15 strains of methicillin-resistant staphylococcus aureus (MRSA) and 1 standard methicillin-susceptible S. aureus (MSSA) strain by using the minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test. Antimicrobial activity of n-hexane fraction of Lysimachia clethroides Duby root was remarkable. Against the 16 strains, the minimum inhibitory concentrations (MICs) were in the range of 31.25–62.5 ㎍/㎖ and FICI values for n-hexane fraction of Lysimachia clethroides Duby root+AM and n-hexane fraction of Lysimachia clethroides Duby root+OX were checkerboard method performed using the MRSA, MSSA and one clinical isolate strains via MICI 0.12-1 and 0.25-0.75, showing the increase of synergistic effect. When combined together, these antibiotic effects were dramatically increased. These effective combinations could be new promising agents in the management of MRSA.

Keywords
Antimicrobial
Lysimachia clethroides Duby
Methicillin-resistant staphylococcus aureus (MRSA)
Synergism
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :719-724
  • Received Date : 2018-08-06
  • Revised Date : 2018-12-03
  • Accepted Date : 2018-12-20
  • DOI :https://doi.org/10.7732/kjpr.2018.31.6.719
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