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2018 Vol.31, Issue 6 Preview Page

Research Article

Effect of Colchicine on the Growth and Gravitropic Response via Ethylene Production in Arabidopsis Roots
Seon Woong Kim1
,
Arom Park1
,
Dong Gyu Ahn1
,
Soon Young Kim2*
1Graduate Student, Department of Biological Sciences, Andong National University
2Professor, Department of Biological Sciences, Andong National University
*Corresponding Author
31 December 2018. pp. 597-603
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Abstract

Inhibitory effect of colchicine on growth and gravitropic responses in Arabidopsis root was explored to find whether there was an involvement of ethylene production. It has been known that cytoskeleton components are implicated in sedimentation of statoliths to respond to gravitropism and growth. The root growth was inhibited by 25% and 40% over control for 8 hr treatment of colchicine at a concentration of 10-5 M and 10-7 M, respectively. The roots treated with colchicine at the concentration of 10-7 M showed the same pattern as control in 3 hr, however, gravitropic response was decreased in the next 5 hr. The colchicine treatment at the concentration of 10-5 M inhibited the gravitropic response resulting in 60° of curvature. In order to better understand the role of colchicine, the production of ethylene was measured with and without the treatment of colchicine. Colchicine increased the ethylene production by 20% when compared to control via the activation of ACC oxidase and ACC synthase activity. These results suggest that the inhibition of the growth and gravitropic responses of Arabidopsis roots by the treatment of colchicine could be attributed to the rearrangement of microtubule, and increase of ethylene production.

Keywords
Arabidopsis root
Colchicine
Ethylene
Gravitropic response
Growth
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :597-603
  • Received Date : 2018-09-04
  • Accepted Date : 2018-11-28
  • DOI :https://doi.org/10.7732/kjpr.2018.31.6.597
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