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2019 Vol.32, Issue 1 Preview Page

Research Article

Optimization of Shoot Induction, Histological Study and Genetic Stability of in vitro Cultured Pisum sativum cv. ‘Sparkle’
Vipada Kantayos1
,
Chang-Hyu Bae23*
1Ph.D. Graduate Student, School of Plant Production Science, Sunchon National University, Suncheon 57922, Korea
2Professor, School of Plant Production Science, Sunchon National University, Suncheon 57922, Korea
3Professor, Department of Well-being Resources, Sunchon National University, Suncheon 57922, Korea
* Corresponding Author
28 February 2019. pp. 19-28
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Abstract

An efficient shoot regeneration condition for pea cv. ‘Sparkle’ was developed by using optimum explant, plant growth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed the highest on two kinds of shoot induction media (MSB5 media containing 2 ㎎/L BA and a combination of 2 ㎎/L BA and 1 ㎎/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 ㎎/L BA solution was found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division and proto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlier than 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitro regenerated plants showed polymorphism with 8.3% compared with their mother plants.

Keywords
Cotyledonary node
Genetic Stability
Histological study
Pea
Shoot Regeneration
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :1
  • Pages :19-28
  • Received Date : 2019-01-07
  • Revised Date : 2019-01-31
  • Accepted Date : 2019-02-10
  • DOI :https://doi.org/10.7732/kjpr.2019.32.1.019
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