All Issue

2018 Vol.31, Issue 3 Preview Page

Research Article

Anti-inflammatory Effects of Abeliophyllum distichum Flower Extract and Associated MAPKs and NF-κB Pathway in Raw264.7 Cells
Jin-Wook Lee1
,
Yoon-Joong Kang1*
1Department of Biomedical Science, Jungwon University, Goesan 28024, Korea
* Corresponding Author
30 June 2018. pp. 202-210
PDF XML Citation
Abstract

Abeliophyllum distichum is a medicinal plant used in regional traditional medicine to relieve pain in inflammatory processes. In this study, anti-inflammatory effects of Abeliophyllum distichum flower (ADF) extract were examined. Furthermore, possible molecular mechanisms of the anti-inflammatory effects were dissected. The anti-inflammatory activity was investigated by inhibition of lipopolysaccharide (LPS) induced pro-inflammatory cytokine production in murine macrophage-like cell line Raw264.7 cells. The measurement of the induced pro-inflammatory cytokine levels were carried out by ELISA. The phosphorylation of ERK1/2, JNK, and MAPK, and the nuclear expression of nuclear factor NF-κB p65 were investigated by Western blot analysis. The extract of ADF significantly decreased the production of pro-inflammatory cytokines. In addition, the extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-κB p65 in activated cells. Our findings provide evidence for the popular use of Abeliophylli distichum in inflammation around Goesan region and also suggest that the flower extract has potential therapeutic benefits against various inflammatory diseases.

Keywords
Abeliophyllum distichum
Anti-inflammatory
Flower extract
NF-κB
References
1
Aderem, A. and R.J. Ulevitch. 2000. Toll-like receptors in the induction of the innate immune response. Nature 406:782-787.
10.1038/3502122810963608
2
Amălinei, C., I.-D. Căruntu and R.A. Bălan. 2007. Biology of metalloproteinases. Rom J Morphol Embryol. 48:323-334.
18060181
3
Axtelle, T. and J. Pribble. 2001. IC14, a CD14 specific monoclonal antibody, is a potential treatment for patients with severe sepsis. J Endotoxin Res. 7:310-314.
10.1179/09680510110153278310.1177/0968051901007004020111717588
4
Bendtzen, K. 1988. Special Issue: Second Meeting on Infection Immunity Elsinore, Denmark, 17-21 June 1988Interleukin 1, interleukin 6 and tumor necrosis factor in infection, inflammation and immunity. Immunol Lett. 19:183-192.
10.1016/0165-2478(88)90141-1
5
Bhattacharyya, A., S. Pathak, S. Datta, S. Chattopadhyay, J. Basu and M. Kundu. 2002. Mitogen-activated protein kinases and NF-kappaB regulate H. pylori-mediated IL-8 release from macrophages. Biochem J. 366:376-382.
6
Binétruy, B., T. Smeal and M. Karin. 1991. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. Nature 351:122-127.
10.1038/351122a01903181
7
Boscá, L., M. Zeini, P.G. Través and S. Hortelano. 2005. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology 208:249-258.
10.1016/j.tox.2004.11.03515691589
8
Coussens, L.M., B. Fingleton and L.M. Matrisian. 2002. Matrix metalloproteinase inhibitors and cancer－trials and tribulations. Science 295:2387-2392.
10.1126/science.106710011923519
9
Farabegoli, F., A. Papi and M. Orlandi. 2011. (–)-Epigallocatechin-3-gallate down-regulates EGFR, MMP-2, MMP-9 and EMMPRIN and inhibits the invasion of MCF-7 tamoxifen-resistant cells. Biosci Rep. 31:99-108.
10.1042/BSR2009014320446926
10
Festuccia, C., D. Giunciuglio, F. Guerra, I. Villanova, A. Angelucci, P. Manduca, A. Teti, A. Albini and M. Bologna. 1998. Osteoblasts modulate secretion of urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) in human prostate cancer cells promoting migration and matrigel invasion. Oncol Res. 11:17-31.
11
Fujiwara, N. and K. Kobayashi. 2005. Macrophages in inflammation. Curr Drug Targets Inflamm Allergy. 4:281-286.
10.2174/156801005402202416101534
12
Ghosh, S. and M.S. Hayden. 2008. New regulators of NF-κB in inflammation. Nat Rev Immunol. 8:837-848.
10.1038/nri242318927578
13
Grivennikov, S.I., F.R. Greten and M. Karin. 2010. Immunity, inflammation, and cancer. Cell 140:883-899.
10.1016/j.cell.2010.01.02520303878PMC2866629
14
Guo, H.-F. and M.-H. Wang. 2017. Anti-inflammatory and anti-cancer effect of Stachys affinis tubers. Korean J. Plant Res. 30:679-685.
15
Herlaar, E. and Z. Brown. 1999. p38 MAPK signalling cascades in inflammatory disease. Mol Med Today. 5:439-447.
10.1016/S1357-4310(99)01544-0
16
Hibi, M., K. Nakajima and T. Hirano. 1996. IL-6 cytokine family and signal transduction: a model of the cytokine system. J Mol Med. 74:1-12.
10.1007/BF002020688834766
17
Hoshino, K., O. Takeuchi, T. Kawai, H. Sanjo, T. Ogawa, Y. Takeda, K. Takeda and S. Akira. 1999. Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product. J Immunol. 162:3749-3752.
18
Hung, W.-C., W.-L. Tseng, J. Shiea and H.-C. Chang. 2010. Skp2 overexpression increases the expression of MMP-2 and MMP-9 and invasion of lung cancer cells. Cancer Lett. 288:156-161.
10.1016/j.canlet.2009.06.03219625121
19
Ide, N. and B.H. Lau. 2001. Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-κB activation. J Nutr. 131:1020S-1026S.
10.1093/jn/131.3.1020S11238809
20
Iwalewa, E., L. McGaw, V. Naidoo and J. Eloff. 2007. Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. Afri J Biotech. 6(25):2868-2885.
10.5897/AJB2007.000-2457
21
Johnson, G.L. and R. Lapadat. 2002. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298:1911-1912.
10.1126/science.107268212471242
22
Lazarov, S., M. Balutsov and E. Ianev. 1999. The role of bacterial endotoxins, receptors and cytokines in the pathogenesis of septic (endotoxin) shock. Vutr Boles. 32:33-40.
23
Lee, E.-J., S.-Y. Kim, J.-W. Hyun, S.-W. Min, D.-H. Kim and H.-S. Kim. 2010. Glycitein inhibits glioma cell invasion through down-regulation of MMP-3 and MMP-9 gene expression. Chem Biol Interact. 185:18-24.
10.1016/j.cbi.2010.02.03720188714
24
Lee, T.H., H.B. Kwak, H.-H. Kim, Z.H. Lee, D.K. Chung, N.-I. Baek and J. Kim. 2007. Methanol extracts of Stewartia koreana inhibit cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression by blocking NF-kappaB transactivation in LPS-activated RAW 264.7 cells. Mol Cells. 23:398-404.
25
Li, Q. and I.M. Verma 2002. NF-κB regulation in the immune system. Nat Rev Immunol. 2:725-734.
10.1038/nri91010.1038/nri96812360211
26
Libby, P., P.M. Ridker and A. Maseri. 2002. Inflammation and atherosclerosis. Circulation 105:1135-1143.
10.1161/hc0902.10435311877368
27
Ljung, T., S. Lundberg, M. Varsanyi, C. Johansson, P.T. Schmidt, M. Herulf, J.O. Lundberg and P.M. Hellstrom. 2006. Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders. World J Gastroenterol. 12:3386.
10.3748/wjg.v12.i21.338616733856PMC4087870
28
Majdalawieh, A. and H.-S. Ro. 2010. Regulation of IkappaBalpha function and NF-kappaB signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediators Inflamm. 2010:823821.
10.1155/2010/82382120396415PMC2855089
29
Nair, V.D., T. Yuen, C.W. Olanow and S.C. Sealfon. 2004. Early single cell bifurcation of pro-and antiapoptotic states during oxidative stress. J Biol Chem. 279:27494-27501.
10.1074/jbc.M31213520015078887
30
Nalla, A.K., B. Gorantla, C.S. Gondi, S.S. Lakka and J.S. Rao. 2010. Targeting MMP-9, uPAR, and cathepsin B inhibits invasion, migration and activates apoptosis in prostate cancer cells. Cancer Gene Ther. 17:599-613.
10.1038/cgt.2010.1620448670PMC2919627
31
Natarajan, K., S.K. Manna, M.M. Chaturvedi and B.B. Aggarwal. 1998. Protein tyrosine kinase inhibitors block tumor necrosis factor-induced activation of nuclear factor-κB, degradation of IκBα, nuclear translocation of p65, and subsequent gene expression. Arch Biochem Biophys. 352:59-70.
10.1006/abbi.1998.05769521814
32
Opal, S.M. and V.A. DePalo. 2000. Anti-inflammatory cytokines. Chest 117:1162-1172.
10.1378/chest.117.4.116210767254
33
Park, G.H., J.H. Park, H.J. Eo, H.M. Song, M.H. Lee, J.R. Lee and J.B. Jeong. 2014. Anti-inflammatory effect of the extracts from Abeliophyllum distichum Nakai in LPS-stimulated RAW264. 7 cells. Korean J. Plant Res. 27:209-214.
10.7732/kjpr.2014.27.3.209
34
Park, J.-H., B.-G. Lee, G.-I. Byun and D.-W. Kim. 2010. Antioxidant activities and inhibitory effect on oxidative DNA damage of Nelumbinis semen extracts. Korean J. Herbol. 25:55-59.
35
Pasparakis, M., L. Alexopoulou, V. Episkopou and G. Kollias. 1996. Immune and inflammatory responses in TNF alpha-deficient mice: a critical requirement for TNF alpha in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response. J Exp Med. 184:1397-1411.
10.1084/jem.184.4.13978879212
36
Ramos-DeSimone, N., E. Hahn-Dantona, J. Sipley, H. Nagase, D.L. French and J.P. Quigley. 1999. Activation of matrix metalloproteinase-9 (MMP-9) via a converging plasmin/stromelysin-1 cascade enhances tumor cell invasion. J Biol Chem. 274:13066-13076.
10.1074/jbc.274.19.1306610224058
37
Ryan, G.B. and G. Majno. 1977. Acute inflammation. A review. Am J Pathol. 86:183.
64118PMC2032041
38
Sil, H., T. Sen and A. Chatterjee. 2011. Fibronectin‐Integrin (α5β1) Modulates migration and invasion of murine melanoma cell line B16F10 by involving MMP-9. Oncol Res. 19:335-348.
10.3727/096504011X1307969713292521936403
39
Vallabhapurapu, S. and M. Karin. 2009. Regulation and function of NF-κB transcription factors in the immune system. Annul Rev Immunol. 27:693-733.
10.1146/annurev.immunol.021908.13264119302050
40
Visse, R. and H. Nagase. 2003. Matrix metalloproteinases and tissue inhibitors of metalloproteinases structure, function, and biochemistry. Circ Res. 92:827-839.
10.1161/01.RES.0000070112.80711.3D12730128
41
Xin, W., L. Wen-bo, L. Yan, Z. Jian and C. Ting-mei. 2013. Effect of osteoprotegerin in combination with interleukin-6 on inhibition of osteoclast differentiation. Chin J Traumatol. 16:277-280.
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :3
  • Pages :202-210
  • Received Date : 2018-03-26
  • Revised Date : 2018-06-13
  • Accepted Date : 2018-06-14
  • DOI :https://doi.org/10.7732/kjpr.2018.31.3.202
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close