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2018 Vol.31, Issue 3 Preview Page

Research Article

Antioxidant Activity and Inhibitory Effects on Oxidative DNA Damage of Callus from Abeliophyllum distichum Nakai
Tae Won Jang1
,
Jae Ho Park2*
1Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Department of Medicinal Plant Science, Jungwon University, Goesan-gun 28024, Korea
* Corresponding Author
30 June 2018. pp. 228-236
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Abstract

In this study, we evaluated the antioxidant activity and protective effects against oxidative DNA damage of the ethyl acetate fraction from the callus of Abeliophyllum distichum Nakai (ECA). Callus of A. distichum was induced on MS medium containing NAA (1 ㎎/L) and 2,4-D (1 ㎎/L), and a sufficient amount was obtained for the extraction by subculture. Acteoside was analyzed and quantified (0.39 ㎎/g callus) from ECA using the high-performance liquid chromatography- photodiode array detector method. ECA showed very high antioxidative activity as revealed by DPPH and ABTS scavenging assays. The IC50 values were 12.4 and 6.8 ㎍/㎖, respectively. ECA showed protective effects against oxidative DNA damage evaluated by using ψX-174 RF I plasmid DNA. It also inhibited DNA damage by suppressing the oxidative stress-induced protein and mRNA levels of γ-H2AX and p53 in NIH/3T3 cells. In conclusion, ECA protects against oxidative DNA damage through its powerful antioxidant activity.

Keywords
Abeliophyllum distichum Nakai
Antioxidant
Callus
DNA damage
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :3
  • Pages :228-236
  • Received Date : 2018-05-11
  • Revised Date : 2018-06-11
  • Accepted Date : 2018-06-18
  • DOI :https://doi.org/10.7732/kjpr.2018.31.3.228
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