All Issue

2020 Vol.33, Issue 5 Preview Page

Research Article

Construction of Data System on Seed Morphological Traits and Functional Component in Tartary Buckwheat Germplasms

쓴메밀 유전자원의 종자특성과 유용성분 변이에 관한 자원 정보 구축

Su Jeong Kim1**
,
Hwang Bae Sohn1**
,
Su Young Hong1
,
Jong Nam Lee1
,
Ki Deog Kim1
,
Jong Taek Suh2
,
Jeong Hwan Nam1
,
Dong Chil Chang3
,
Min Woo Park4
,
Yul Ho Kim2*
김 수정1**
,
손 황배1**
,
홍 수영1
,
이 종남1
,
김 기덕1
,
서 종택2
,
남 정환1
,
장 동칠3
,
박 민우4
,
김 율호2*
1Researcher, Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea
2Senior Researcher, Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea
3Researcher, Korea Program for International Cooperation in Agricultural Technology, Technology Cooperation Bureau, Rural Development Administration, Jeonju 54875, Korea
4Researcher, Hyundai Seed Co. Ltd, Yeoju 12660, Korea
1농촌진흥청 국립식량과학원 고령지농업연구소, 농업연구사
2농촌진흥청 국립식량과학원 고령지농업연구소, 농업연구관
3농촌진흥청 기술협력국 국외농업기술과, 농업연구사
4농업회사법인 현대종묘㈜, 연구원
* Corresponding Author
** First Author
1 October 2020. pp. 446-459
PDF XML Citation
Abstract

This study analyzed the phenotypes and chemotypes of 74 tartary buckwheat (Fagopyrum tataricum) germplasms using principal component analysis and cluster analysis. The average seed size of tartary buckwheat germplasm was 5.2 × 3.4 ㎜, which is smaller than the seed size of common buckwheat. The dark browned colored ovate or elliptic shape was mostly observed in collected germplasm. The average content of rutin was 1,393 ㎎ per 100 g dry weight (DW) in tartary buckwheat seed. Similarly, the flavonoid and polyphenol contents ranged from 253 to 2,669 and 209 to 1,823 ㎎, respectively, per 100 g DW in the collected germplasm. The three components (PC1, 2, and 3) of principal component analysis revealed 68.55% of the total variance of the collected accessions. Cluster analysis using descriptors showed that 74 accessions were clustered into five groups. The study showed that the most interesting resources for functional breeding programs are: Five resources (HLB1004, HLB1005, HLB1007, HLB1009, and HLB1013) due to the rich rutin, polyphenol, and flavonoid.

Keywords
Flavonoid
Germplasm
Polyphenol
Rutin
Seed
References
1
Ahmed, A., N. Khalid, A. Ahmad, N.A. Abbasi, M.S.Z. Latif and M.A. Randhawa. 2013. Phytochemical and bio functional properties of buckwheat: A review. J. Agric. Sci. 152:349-369.
10.1017/S0021859613000166
2
Ahn, M.S., P.H. Park, Y.N. Kwon, M. Mekapogu, S.W. Kim, E.Y. Jie, J.A. Jeong, J.T. Park and O.K. Kwon. 2018. Discrimination of floral scents and metabolites in cut flowers of peony (Paeonia lactiflora Pall.) cultivars. Korean J. Plant Res. 31:641-651.
3
Baniya, B.K., D.M.S. Dongol and N.R. Dhungel. 1995. Further characterization and evaluation of Nepalese buckwheat (Fagopyrum spp.) landraces: In Matano, T. and A. Ujihara (eds.), Current Advances in Buckwheat Research, Proc. 6th Int. Symp. on Buckwheat in Shinshu, 24-29 August, 1995 Shinshu University Press, Japan. Vol. I-III. pp. 295-304.
4
Bonafaccia, G., L. Gambelli, N. Fabjan and I. Kreft. 2003. Trace elements in flour and bran from common and tartary buckwheat. Food Chem. 83:1-5.
10.1016/S0308-8146(03)00228-0
5
Campbell, C.G. 1997. Buckwheat Fagopyrum esculentum Moench. International Plant Genetic Resources Institute, CAB International. pp. 3-93.
6
Chae, Y.A, J.S. Song and M.G. Cho. 2001. Identification of chemotypes in traditional aromatic pant resources Z. schinifolium Siebold et Zucc. and Z. piperitum DC. Korean J. Breed. Sci. 33:126-132.
7
Chang, E.H., K.J. Sa, J.H. Kim and J.K. Lee. 2013. Analysis of morphological characteristics among popcorn inbred lines. Korean J. Crop Sci. 58:267-273.
10.7740/kjcs.2013.58.3.267
8
Chao, P.L., S. Hsiu and Y. Hou. 2002. Flavonoids in herbs: Biological fates and potential interactions with xenobiotics. J. Food Drug Anal. 10:219-228.
9
Chauhan, R.S., N. Gupta, S.K. Sharma, J.C. Rana, T.R. Sharma and S. Jana. 2010. Genetic and genome resources in buckwheat - present status and future perspectives. Eur. J. Plant Sci. Biotechnol. 4:33-44.
10
Chen, Q.F., X.Y. Huang, H.Y. Li, L.J. Yang and Y.S. Cui. 2018. Recent progress in perennial buckwheat development. Sustainability 10:536.
10.3390/su10020536
11
Dewanto, V, W. Xianzhong and R.H. Liu. 2002. Processed sweet corn has higher antioxidant activity. J. Agr. Food Chem. 50:4959-4964.
10.1021/jf025593712166989
12
Fabjan, N., J. Rode, I.J. Kosir, Z. Zhang and I. Kreft. 2003. Tartary buckwheat (Fagopyrum tataricm Gaertn.) as a source of dietary rutin and quercitrin. J. Agric. Food Chem. 51:6452-6455.
10.1021/jf034543e14558761
13
Ferguson, A.R. 2007. The need for characterization and evaluation of germplasm: Kiwifruit as and example. Euphytica 154: 371-382.
10.1007/s10681-006-9188-2
14
Folin, O. and W. Denis. 1912. On phosphotungstic phosphomolybdic compounds as color reagents. J. Biol. Chem. 12:239-249.
15
Hertog, M.G.L., D. Kromhout, C. Aravanis, H. Blackburn, R. Buzina, F. Fidanza, S. Giampaoli, A. Jansen, A. Menotti, S. Nedeljkovic, M. Pekkarinen, B.S. Simic, H. Toshima, E.J.M. Feskens, P.C.H. Hollman and M.B. Katan. 1995. Flavonoid intake and long-term risk of coronary heart disease and cancer in seven countries study. Arch. Intern. Med. 155:381-386.
10.1001/archinte.1995.004300400530067848021
16
Holasova, M., V. Fiedlerova, H. Smrcinova, M. Orsak, J. Lachman and S. Vavreinova. 2002. Buckwheat - the source of antioxidant activity in functional foods. Food Res. International 35:207-211.
10.1016/S0963-9969(01)00185-5
17
Huang, X.Y., F.J. Zeller, K.F. Hung, T.X. Shi and Q.F. Chen. 2014. Variation of major minerals and trace elements in seeds of tartary buckwheat (Fagopyrum tataricum Gaertn.). Genet. Res. Crop Evol. 61:567-577.
10.1007/s10722-013-0057-2
18
Hyun, D.Y., M. Rauf, S.K. Lee, H.C. Ko, S.J. Oh, M.C. Lee and Y.M. Choi. 2018. Comparison of growth characteristics and flavonoids content by different cultivation seasons in buckwheat germplasm. Korean J. Plant Res. 31:489-497.
19
Jiang, P., F. Burczynski, C. Campbell, G. Pierce, J.A. Austria and C.J. Briggs. 2007. Rutin and flavonoid contents in three buckwheat species Fagopyrum esculentum, F. tataricum and F. homotropicum and their protective effects against lipid peroxidation. Food Res. Int. 40:356-364.
10.1016/j.foodres.2006.10.009
20
Joshi, B.D. and R.S. Rana. 1995. Buckwheat (Fagopyrum esculentum): In Williams, J.T. (ed.), Cereals and Pseudo Cereals, Champman and Hall, New York, US, pp. 85-127.
21
Keli, Y. 1992. Research on cultivated buckwheat germplasm resources in China: In Lin, R., M.D. Zhou, Y. Tao, J. Li and Z. Zhang (eds.), Proc. 5th Int. Symp. on Buckwheat, 20-26 August 1992, Taiyuan, China, Agricultural Publishing House. pp. 55-59.
22
Khorasani, S.K., K. Mostafavi, E. Zandipour and A. Heidarian, 2011. Multivariate analysis of agronomic traits of new corn hybrids (Zea mays L.). International J. Agri. Sci. 1:314-322.
23
Kim, D.Y., M.K. Yoon, T.I. Kim J.H. Kim and J.H. Kwack. 2009. Classification of strawberry germplasms based on horticultural traits and principal component analysis. Korean J. Hort. Sci. Tech. 27:636-643.
24
Kim, M.S., H.J. Lee, D.A. Yu, J.Y. Song, M. Nino, F. Nogoy, J.K. Kim, Y.S. So and Y.G. Cho. 2016. Classification of Korean rice varieties based on agro-morphological traits. Korean J. Breed. Sci. 48: 254-270.
10.9787/KJBS.2016.48.3.254
25
Kim, S.J., C.H. Lee, J. Kim and K.S. Kim. 2014. Phylogenetic analysis of Korean native Chrysanthemum species based on morphological characteristics. Sci. Horti. 175:278-289.
10.1016/j.scienta.2014.06.018
26
Kim, S.J., H.B. Sohn, G.H. Kim, Y.Y. Lee, S.Y. Hong, K.D. Kim, J.H. Nam, D.C. Chang, J.T. Suh, B.J. Koo and Y.H. Kim. 2017. Comparison and validation of rutin and quercetin contents according to the extraction method of tartary buckwheat (Fagopyrum tataricum Gaertn.). Korean J. Food Sci. Technol. 49:258-264.
27
Kim, S.J. and Y.H. Kim. 2018. Agricultural guide of buckwheat. Rural Development Administration, Jeonju, Korea. pp. 7-90.
28
Kitabayashi, H., A. Ujihara, T. Hirose and M. Minami. 1995. Varietal differences and heritability for rutin content in common buckwheat, Fagopyrum esculentum Moench. Japan J. Breed. 45:75-79.
10.1270/jsbbs1951.45.75
29
Koyama, M., C. Nakamura and K. Nakamura. 2013. Changes in phenols contents from buckwheat sprouts during growth stage. J. Food Sci. Technol. 50:86-93.
10.1007/s13197-011-0316-124425891PMC3550953
30
Kreft, I., G. Bonafacci and A. Zigo. 1994. Secondary metabolites of buckwheat and their importance in human nutrition. Food Technol. Biotech. 32:195-197.
31
Lee, M.H., H.L. Kim, H.C. Rhee, Y.B. Kwack, H.M.P.C. Kumarihami and J.G. Kim. 2020. Evaluation of the genetic resources of kiwifruit with multivariate analysis. Horti. Sci. Tech. 38:569-581.
32
Lee, S.K., H.Y. Yoon, M.C. Lee, S.J. Oh, M. Rauf, O.S. Hur, N.Y. Ro, J.Y. Yi, D.Y. Hyun, G.T. Cho, H.C. Ko and Y.M. Choi. 2019. Comparison of the diversity of east Asian oat (Avena sativa L.) genetic resources by origins, considering major nutritional ingredients and agronomic traits. Korean J. Breed. Sci. 51:9-19.
10.9787/KJBS.2019.51.1.9
33
Li, J., F. Gong and F. Li. 2016. Hypoglycemic and hypolipidemic effects of flavonoids from tartary buckwheat in type 2 diabetic rats. Biomed. Res. 27:132-137.
34
Li, S.Q. and Q.H. Zhang. 2001. Advances in the development of functional foods from Buckwheat. Crit. Rev. Food Sci. Nutr. 41:451-464.
10.1080/2001409109188711592684
35
Liu, C.L., Y.S. Chen, J.H. Yang and B.H. Chiang. 2008. Antioxidant activity of tartary (Fagopyrum tataricum (L.) Gaertn.) and common (Fagopyrum esculentum Moench) buckwheat sprouts. J. Agric. Food Chem. 56:173-178.
10.1021/jf072347s18072736
36
Merendino, N., R. Molinari, L. Costantini, A. Mazzucato, A. Pucci and G. Bonafaccia. 2014. A new 'functional' pasta containing tartary buckwheat sprouts as an ingredient improves the oxidative status and normalizes some blood pressure parameters in spontaneously hypertensive rats. Food Function 5:1017-1026.
10.1039/C3FO60683J24658587
37
Nam, T.G., S.M. Lee, J.H. Park, D.O. Kim, N.I. Baek and S.H. Eom. 2015. Flavonoid analysis of buckwheat sprouts. Food Chem. 170:97-101.
10.1016/j.foodchem.2014.08.06725306322
38
Nielsen, J.P. and L. Munck. 2003. Evaluation of malting barley quality using exploratory data analysis. I. Extraction of information from micromalting data of spring and winter barley. J. Cereal Sci. 38:173-180.
10.1016/S0733-5210(03)00023-7
39
Park, H.S., K.Y. Ha, K.Y. Kim, J.K. Nam, M.K. Baek, J.J. Kim, J.M. Jeong, Y.C. Cho. J.H. Lee, B.K. Kim and S.N. Ahn. 2015. Development of high-yielding rice lines and analysis of panicle and yield-related traits using doubled haploid lines derived from the cross between Deuraechan and Boramchan, high-yielding japonica rice cultivars in Korea. Korean J. Breed. Sci. 47:384-402.
10.9787/KJBS.2015.47.4.384
40
Park, M.W., Y.S. Chung and S.H. Lee. 2016. Quality changes in tomato fruits caused by genotype and environment interaction. Horti. Sci. Technol. 35:361-372.
10.12972/kjhst.20170038
41
Rauf, M., H.Y. Yoon, S.K. Lee, D.Y. Hyun, M.C. Lee, S.J. Oh and Y.M. Choi. 2019. Evaluation of sprout growth traits and flavonoid content in common and tartary buckwheat germplasms. Plant Breed. Biotech. 7:375-385.
10.9787/PBB.2019.7.4.375
42
Rice-Evans, C., N. J. Miller and G. Paganga. 1996. Structure antioxidant activity relationships of flavonoids and phenolic acids. Free Rad. Bio. Med. 20:933-956.
10.1016/0891-5849(95)02227-9
43
R Studio Team. 2019. R Studio: Integrated development for R. R Studio, Inc., Boston, Massachusetts, U.S.A. URL http://www.rstudio.com.
44
Sung, J.S., J.B. Kim, H.N. Yoon, J.W. Jeong, J.H. Park, H.J. Baek, J.H. Lee, C.G. Park, M.K. Huh and G.T. Cho. 2011. Comparison of plant morphological traits and fatty acids by GC-MS in collected genetic resources of Carthamus tinctorius L. Korean J. Intl. Agri. 23:306-314.
45
SAS. 2019. SAS User's guide. Statistics, Version 9.4, SAS Institute Inc, Cary NC.
46
Tsuji, K. and O. Ohnishi. 2001. Phylogenetic relationships among wild and cultivated tartary buckwheat (Fagopyrum tataricum Gaert.) populations revealed by AFLP analyses. Genes Genetic Systems 76:47-52.
10.1266/ggs.76.4711376551
47
Wang, L., X. Yang, P. Qin, F. Shan and G. Ren. 2013. Flavonoid composition, antibacterial and antioxidant properties of tartary buckwheat bran extract. Ind. Crop. Prod. 49:312-317.
10.1016/j.indcrop.2013.04.039
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :5
  • Pages :446-459
  • Received Date : 2020-07-27
  • Revised Date : 2020-09-04
  • Accepted Date : 2020-09-06
  • DOI :https://doi.org/10.7732/kjpr.2020.33.5.446
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close