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2018 Vol.31, Issue 3

Research Article

In Vitro Cytotoxicity against Human Cancer Cell and 3T3-L1 Cell, Total Polyphenol Content and DPPH Radical Scavenging of Codonopsis lanceolata according to the Concentration of Ethanol Solvent
Hee-Ock Boo1*
,
Jeong-Hun Park1
,
Moon-Soon Lee2
,
Soo-Jeong Kwon3
,
Hag-Hyun Kim3
1WELLPHYTO Co. Ltd., BI Center, GIST, Gwangju 61005, Korea
2Department of Industrial Plant Science & Technology, Chungbuk National University, Cheongju 28644, Korea
3Faculty of Food Nutrition and Cookery, Woosong Information College, Daejeon 34606, Korea
* Corresponding Author
30 June 2018. pp. 195-201
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Abstract

This study was executed to evaluate the phenolic content, DPPH radical scavenging rate, and the cytotoxic effect in human cancer cell, 3T3-L1 cell from C. lanceolata extracts at various ethanol concentration. Total polyphenol and flavonoid content of the C. lanceolata at various ethanol concentration showed the high amount in 70%, 100% ethanol extract. The DPPH radical scavenging activity progressively increased in a dose-dependent manner, and showed the highest in 100% ethanol extract. The cytotoxic effect against human cancer cell of the C. lanceolata was higher in 50% and 70% ethanol extracts. In particular, the cytotoxic effect in MCF-7 cell was relatively higher than in other cells. The IC50 (concentration causing 50% cell death) value showed the highest on MCF-7 cell (538.39 ㎍/㎖) in 70% ethanol extract, and exhibited significant activity against Hela cell (637.87 ㎍/㎖), Calu-6 cell (728.64 ㎍/㎖). The extract of 70% ethanol at 1,000 ㎍/㎖ exhibited a pronounced cytotoxic effect on 3T3-L1 cell comparable to that of the other extracts, and reduced in a concentration-dependent manner.

Keywords
Codonopsis lanceolate
Cytotoxicity
DPPH radical
Flavonoid
Polyphenol
3T3-L1 preadipocyte cell
References
1
Boo, H.O., J.H. Shin, J.S. Shin, E.S. Choung, M.A. Bang, K.M. Choi and W.S. Song. 2012. Assessment on antioxidant potential and enzyme activity of some economic resource plants. Korean J. Plant Res. 25(3):349-356.
10.7732/kjpr.2012.25.3.349
2
Boo, H.O., J.H. Park, H.G. Kim, H.H. Kim, S.J. Kwon, D.Y. Seo and M.S. Lee. 2016. Immune cells activity, nitrite scanvenging and ABTS radical scavenging activities of Codonopsis lanceolata ethanol extracts from districts in Korea. Korean J. Plant Res. 29(3):289-296.
10.7732/kjpr.2016.29.3.289
3
Brand-Williams, W., M.E. Cuvelier and C. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Tech. 28:25-30.
10.1016/S0023-6438(95)80008-5
4
Cao, H., M.M. Polansky and R.A. Anderson. 2007. Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes. Arch Biochem Biophys. 459(2):214-222.
10.1016/j.abb.2006.12.034
5
Cuyacot, A.R., J.J.M. Mahilum and M.R.S.B. Madamba. 2014. Cytotoxicity potentials of some medicinal plants in Mindanao, Philippines. Pelagia Research Library 4(1):81-89.
6
Ejaz, A., D. Wu, P. Kwan and M. Meydani. 2009. Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice. The Journal of Nutririon(JN) 139(5):919-925.
10.3945/jn.108.100966
7
Fu, X.J., H.B. Liu, P. Wang and H.S. Guan. 2009. A study on the antioxidant activity and tissues selective inhibition of lipid peroxidation by saponins from the roots of Platycodon grandiflorum. Am. J. Chin. Med. 37(5):967-975.
10.1142/S0192415X09007375
8
Guizani, N., M.I. Waly, V. Singh and M.S. Rahman. 2013. Nabag (Zizyphus spina-christi) extract prevents aberrant crypt foci development in colons of azoxymethane-treated rats by abrogating oxidative stress and inducing apoptosis. Asian Pac J Cancer Prev. 14:5031-5035.
10.7314/APJCP.2013.14.9.503124175771
9
Joh, E.H., I.A. Lee, S.J. Han, S.J. Chae and D.H. Kim. 2010. Lancemaside A ameliorates colitis by inhibiting NF-jB activation in TNBS-induced colitis mice. Int. J. Colorectal Dis. 25:545-551.
10.1007/s00384-009-0858-019956958
10
Kim, C.H., B.Y. Jung, S.K. Jung, C.H. Lee, H.S. Lee, B.H. Kim and S.K. Kim. 2010. Evaluation of antioxidant activity of Platycodon grandiflorum. J. Environ. Toxicol. 25(1):85-94.
11
Kim, I.D., S.K. Dhungana, H.R. Kim and D.H. Shin. 2017. Quality characteristics and antioxidant potential of seeds of native Korean persimmon genotypes. Korean J. Plant Res. 30(6):670-678.
12
Kintzios, E. 2006. Terrestrial plant-derived anticancer agents and plant species used in anticancer research. Crit. Rev. Plant Sci. 25:79-113.
10.1080/07352680500348824
13
Li, X., Y. Huang, L. Zheng, H. Liu, X. Niu, J. Huang, F. Zhao and Y. Fan. 2014. Effect of substrate stiffness on the functions of rat bone marrow and adipose tissue derived mesenchymal stem cells in vitro. J. Biomed. Mater. Res. 102(4):1092-1101.
10.1002/jbm.a.3477423630099
14
Lobo, R., K.S. Prabhu and A. Shirwaikar. 2009. Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties. J. Pharm. Pharmacol. 61:13-21.
10.1211/jpp/61.01.000310.1211/jpp.61.01.000319126292
15
Marja, P.K., I.H. Anu, J.V. Heikki, R. Jussi-Pekka, P. Kalevi, S.K. Tytti and H. Marina. 1999. Antioxidant activity of plant extracts containing phenolic compounds. J. Agri. Food Chem. 47:3954-3962.
10.1021/jf990146l
16
Park, H.J., M.J. Kim, E. Ha and J.H. Chung. 2008. Apoptotic effect of hesperidin through caspase 3 activation in human colon cancer cells, SNU-C4. Phytomedicine 15:147-151.
10.1016/j.phymed.2007.07.06117897817
17
Rahul, B.B. and K.B. Kamlesh. 2007. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov. Today 12:879-889.
10.1016/j.drudis.2007.07.02417933690
18
Rice-Evans, C., N.J. Miller, G.P. Bolwell, P.M. Bramley and J.B. Pridham. 1995. The relative antioxidants activities of plant-derived polyphenolic flavonoids. Free Radic. Res. 22:375-383.
10.3109/107157695091456497633567
19
Roh, C.H. and U.H. Jung. 2012. Screening of crude plant extracts with anti-obesity activity. Int. J. Mol. Sci. 13(2):1710-1719.
10.3390/ijms1302171022408418PMC3291987
20
Ryu, C.S., C.H. Kim, S.Y. Lee, K.S. Lee, K.J. Choung, G.Y. Song, B.H. Kim, S.Y. Ryu, H.S. Lee and S.K. Kim. 2012. Evaluation of the total oxidant scavenging capacity of saponins isolated from Platycodon grandiflorum. Food Chem. 132(1):333-337.
10.1016/j.foodchem.2011.10.08626434298
21
Sanchez-Moreno, C., J.A. Larrauri and F. Saura-Calixto. 1999. Free radical scavenging capacity and inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Res. Int. 32:407-412.
10.1016/S0963-9969(99)00097-6
22
Shahidi, F. and P.K.J.P.D. Wanasundara. 1992. Phenolic antioxidants: criteria review. Food Science and Nutrition 32:67-103.
10.1080/10408399209527581
23
Singleton, V. and J.A. Rossi. 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am. J. Enology Vitic. 16:144-158.
24
Sugihara, N., T. Arakawa, M. Ohnishi and K. Furuno. 1999. Anti and pro-oxidative effects of flavonoids on metal induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes located with "- linolenic acid. Free Radic. Biol. Med. 27:1313-1323.
10.1016/S0891-5849(99)00167-7
25
Velioglu, Y.S., G. Mazza, L. Gao and B.D. Oomah. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food. Chem. 46:4113-4117.
10.1021/jf9801973
26
Vijayarathna, S. and S. Sasidharan. 2012. Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines. Asian Pac. J. Trop Biomed. 2(10):826-829.
10.1016/S2221-1691(12)60237-8
27
Wang, L., M.L. Xu, J.H.u, S.K. Rasmussen and M.H. Wang. 2011. Codonopsis lanceolata extract induces G0/G1 arrest and apoptosis in human colon tumor HT-29 cells – Involvement of ROS generation and polyamine depletion. Food Chem. Toxicol. 49:149-154.
10.1016/j.fct.2010.10.01020940027
28
Yan-Wei, H., L. Chun-Yu, D. Chong-Min, W. Wen-Qian and G. Zhen-Lun. 2009. Induction of apoptosis in human hepatocarcinoma SMMC-7721 cells in vitro by flavonoids from Astragalus complanatus. J. Ethnopharmacol. 123:293-301.
10.1016/j.jep.2009.03.01619429375
29
Yokozawa, T., C.P. Chen, E. Dong, T. Tanaka, G.I. Nonaka and I. Nishioka. 1998. Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical. Biochem. Phamacol. 56:213-222.
10.1016/S0006-2952(98)00128-2
30
Zhishen, J., T. Mengcheng and W. Jianming. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64:555-559.
10.1016/S0308-8146(98)00102-2
31
Zhou, K. and L. Yu. 2006. Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado. LWT . 39:1155-1162.
10.1016/j.lwt.2005.07.015
32
Zhu, N., M. Wang, G.J. Wei, J.K. Lin, C.S. Yang and C.T. Ho. 2001. Identification of reaction products of (-)-epigallocatechin, (-)-epigallocatechin gallate and pyrogallol with 2,2-diphenyl-1-picrylhydrazyl radical. Food Chem. 73:345-349.
10.1016/S0308-8146(00)00308-3
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :3
  • Pages :195-201
  • Received Date : 2018-06-04
  • Revised Date : 2018-06-14
  • Accepted Date : 2018-06-14
  • DOI :https://doi.org/10.7732/kjpr.2018.31.3.195
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