Proteome Characterization of Sorghum (Sorghum bicolor L.) at Vegetative Stage under Waterlogging Stress

Min Heon Yun; Hae-Ryong Jeong; Jang-Hwan Yoo; Roy Swapan Kumar; Soo-Jeong Kwon; Joo-Ho Kim; Hyen Chung Chun; Ki Yuol Jung; Seong-Woo Cho; Sun-Hee Woo

doi:10.7732/kjpr.2018.31.2.124

All Issue

2018 Vol.31, Issue 2 Preview Page Next Page

Research Article

Proteome Characterization of Sorghum (Sorghum bicolor L.) at Vegetative Stage under Waterlogging Stress 토양 과습 조건하에서 수수 잎의 단백질 양상

30 April 2018. pp. 124-135

PDF XML

Abstract

The study was performed to explore the molecular changes in the vegetative stage (3-and 5-leaf) of sorghum under waterlogging stress. A total of 74 differentially expressed protein spots were analyzed using LTQ-FT-ICR MS. Among them, 12 proteins were up-regulated and 3 proteins were down-regulated. Mass spectrometry (MS) results showed that about 50% of the proteins involved in various metabolic processes. The level of protein expression of malate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase related to carbohydrate metabolic process increased in both 3 and 5-leaf stage under waterlogging stress. These proteins are known to function as antistress agents against waterlogging stress. The expression of oxygen-evolving enhancer protein 1 protein related to photosynthesis was slightly increased in the treated group than in the control group, however the expression level was increased in the 5-leaf stage compared to the 3-leaf stage. Probable phospholipid hydroperoxide glutathione peroxidase protein and superoxide dismutase protein related to response to oxidative stress showed the highest expression level in 5-leaf stage treatment. This suggests that the production of reactive oxygen species by the waterlogging stress was the most abundant in the 5-leaf treatment group, and the expression of the antioxidant defense protein was increased.

Keywords

Functional characterization

Proteome

Sorghum

Waterlogging stress

References

Ahmed, S., E. Nawata, M. Hosokawa, Y. Domae and T. Sakuratani. 2002. Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Sci. 163(1):117–123.

10.1016/S0168-9452(02)00080-8

Ahsan, N., D.G. Lee, S.H. Lee, K.Y. Kang, J.D. Bahk, M.S. Choi and B.H. Lee. 2007. A comparative proteomic analysis of tomato leaves in response to waterlogging stress. Physiol. Plant. 131(4):555–570.

10.1111/j.1399-3054.2007.00980.x18251847

Bowler, C., M.V. Montagu and D. Inze. 1992. Superoxide dismutase and stress tolerance. Annu. Rev. Plant Biol. 43(1):83–116.

10.1146/annurev.pp.43.060192.000503

Crawford, R.M.M. 1978. Metabolic adaptation to anoxia: In Hook, D.D. and R.M.M. Crawford (eds.), Plant Life in Anaerobic Environments. Ann Arbor, MI: Ann Arbor Science pp. 119-136.

Daugherty, C.J. and M.E. Musgrave. 1994. Characterization of populations of rapid-cycling Brassica rapa L. selected for differential waterlogging tolerance. J Exp. Bot. 8(3):385- 392.

10.1093/jxb/45.3.385

Hoganson, C.W. and G.T. Babcock. 1997. A metalloradical mechanism for the generation of oxygen from water in photosynthesis. Science 277(5334):1953-1956.

10.1126/science.277.5334.19539302282

Kim, H.R. 2014. Differential Expression of Proteins from Diploid and Tetraploid Tissue Culture of Platycodon grandiflorum. Department of Agronomy, MS Thesis, Chungbuk National Univ., Korea. pp. 3-8 (in Korean).

Kim, K.A., H.M. Kamal, K.H. Shin, J.S. Choi, H.Y. Heo and S.H. Woo. 2010. Large-scale proteomics investigation in wild relatives (A, B, D genomes) of wheat. Acta Biochim. Biophys. Sin. 42(10):709-716.

10.1093/abbs/gmq07920810536

Komatsu, S., Y. Nanjo and M. Nishimura. 2013. Proteomic analysis of the flooding tolerance mechanism in mutant soybean. J Proteomics 79:231-250.

10.1016/j.jprot.2012.12.02323313221

Pandey, A. and M. Mann. 2000. Proteomics to study genes and genomes. Nature 10(6788):837-846.

10.1038/3501570910866210

Rabilloud, T. 2002. Two-dimensional gel electrophoresis in proteomics: Old, old fashioned, but it still climbs up the mountains. Proteomics 8(1):3-10.

10.1002/1615-9861(200201)2:1< 3::AID-PROT3>3.0.CO;2-R

Rocha, M., F. Licausi, W.L. Araújo, A. Nunes-Nesi, L. Sodek, A.R Fernie and J.T. van Dongen. 2010. Glycolysis and the tricarboxylic acid cycle are linked by alanine aminotransferase during hypoxia induced by waterlogging of Lotus japonicus. Plant Physiol. 152(3):1501-1513.

10.1104/pp.109.15004520089769PMC2832266

Sugimoto, M. and K. Takeda. 2003. Structure and function of a phospholipid hydroperoxide glutathione peroxidase-like protein from barley. Journal of Molecular Catalysis B: Enzymatic 23(2):397-403.

10.1016/S1381-1177(03)00104-8

Woo, S.H., H.S. Kim, B.H. Song, C.W. Lee, Y.M. Park, S.K. Jong and Y.G. Cho. 2003. Rice proteomics: A functional analysis of the rice genome and applications. J Plant Biotechnol. 11(3):281-291 (in Korean).

Yachandra, V.K., V.J. DeRose, M.J. Latimer, I. Mukerji, K. Sauer and M.P. Klein. 1993. Where plants make oxygen - a structural model for the photosynthetic oxygen-evolving manganese cluster. Science 5(5108):675-679.

10.1126/science.8480177

Yoon, S.T., I.H. Jeong, T.K. Han, Y.J. Kim, J.B. Yu, G. Yang, M.H. Ye, S.W. Baek and K.W. Kim. 2016. Evaluation of crop characteristics of sorghum (Sorghum bicolor L.) germplasm for the selection of excellent resources. Korean J. Plant Res. 29(4):479-494 (in Korean).

10.7732/kjpr.2016.29.4.479

Information

Publisher :The Plant Resources Society of Korea
Publisher(Ko) :한국자원식물학회
Journal Title :Korean Journal of Plant Resources
Journal Title(Ko) :한국자원식물학회지
Volume : 31
No :2
Pages :124-135
Received Date : 2017-10-30
Revised Date : 2018-01-26
Accepted Date : 2018-03-13
DOI :https://doi.org/10.7732/kjpr.2018.31.2.124

Korean Journal of Plant Resources ISSN:1226-3591(Print) 2287-8203(Online) 한국자원식물학회지

All Issue