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2018 Vol.31, Issue 3 Preview Page

Research Article

Anticancer Activity of the Branch Extracts from Vaccinium oldhamii through Cyclin D1 Proteasomal Degradation in Human Cancer Cells
Su Bin Park1
,
Ha Na Kim1
,
Gwang Hun Park2
,
Ho-Jun Son2
,
Hyun Ji Eo2
,
Jeong Ho Song2
,
Hun Min Song3
,
Ji Ae Park3
,
Jin Boo Jeong1*
1Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
3Baekdudaegan National Arboretum, Bonghwa 36209, Korea
* Corresponding Author
30 June 2018. pp. 218-227
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Abstract

In this study, we investigated the effect of the extracts from Vaccinium oldhamii on cell proliferation and the regulatory mechanisms of cyclin D1 protein level in human cancer cells. The branch extracts from Vaccinium oldhamii (VOB) showed higher inhibitor effect against the cell growth than leave extracts (VOL) and fruit extracts (VOF) in human colorectal cancer, breast cancer, prostate cancer, non-small lung cancer, pancreatic cancer and liver cancer cells. In addition, VOB decreased cyclin D1 level at both protein and mRNA level. MG132 treatment attenuated VOB-mediated cyclin D1 downregulation. A point mutation of threonine-286 to alanine attenuated cyclin D1 degradation by VOB. In addition, the inhibition of nuclear export by leptomycin B (LMB) attenuated cyclin D1 degradation by VOB. But, the treatment of PD98059 (ERK1/2 inhibitor), SB203580 (p38 inhibitor), SP600125 (JNK inhibitor), LiCl (GSK3β inhibitor), LY294002 (PI3K inhibitor) or BAY 11-7082 (IκK inhibitor) did not affect VOB-induced cyclin D1 degradation. In conclusion, VOB induced cyclin D1 degradation through redistribution of cyclin D1 from the nucleus to cytoplasm via T286 phosphorylation of cyclin D1, which resulted in the inhibition of cancer cell proliferation.

Keywords
Chemoprevention
Chemotherapy
Functional plant resources
Proteasomal degradation
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :3
  • Pages :218-227
  • Received Date : 2018-05-29
  • Revised Date : 2018-06-11
  • Accepted Date : 2018-06-12
  • DOI :https://doi.org/10.7732/kjpr.2018.31.3.218
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