• Research Article

    Inhibitory Effect of the Branch Extracts from Taxillus yadoriki Parasitic to Neolitsea sericea against the Cell Proliferation in Human Lung Cancer Cells, A549

    참식나무(Neolitsea sericea) 기주 참나무겨우살이(Taxillus yadoriki) 가지 추출물의 폐암세포 A549에 대한 세포생육 억제활성

    Su Bin Park, Ha Na Kim, Jeong Dong Kim, Gwang Hun Park, Hyun Ji Eo, Mi-Yun An and Jin Boo Jeong

    박수빈, 김하나, 김정동, 박광훈, 어현지, 안미연, 정진부

    In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of branches from Taxillus yadoriki parasitic ... + READ MORE
    In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of branches from Taxillus yadoriki parasitic to Neolitsea sericea (TN-NS-B) against human lung cancer cells, A549. TY-NS-B dose-dependently suppressed the growth of A549 cells. TY-NS-B decreased β-catenin protein level, but not mRNA level in A549 cells. The downregulation of β-catenin protein level by TY-NS-B was attenuated in the presence of MG132. Although TY-NS-B phosphorylated β-catenin protein, the inhibition of GSK3β by LiCl did not blocked the reduction of β-catenin by TY-NS-B. In addition, TY-NS-B decreased β-catenin protein in A549 cells transfected with Flag-tagged wild type β-catenin or Flag-tagged S33/S37/T41 mutant β-catenin construct. Our results suggested that TN-NS-B may downregulate β-catenin protein level independent on GSK3β-induced β-catenin phosphorylation. Based on these findings, TY-NS-B may be a potential candidate for the development of chemopreventive or therapeutic agents for human lung cancer. - COLLAPSE
    April 2019
  • Research Article

    Analysis of Index Component Content and Antioxidant Activity According to the Root Diameter of Angelica gigas Nakai

    참당귀 뿌리 직경별 지표성분 함량 및 항산화 활성 분석

    Sang-Hoon Lee, So-Hee Lee, Meilan Jin, Chung-Oui Hong, Mok Hur, Jong-Won Han, Woo-Moon Lee, Hyeong Muk Yun, Yeon Bok Kim, Yi Lee and Sung Cheol Koo

    이상훈, 이소희, 김미란, 홍충의, 허 목, 한종원, 이우문, 윤형묵, 김연복, 이 이, 구성철

    Angelica gigas Nakai (AGN) is a traditional medicinal herb especially in Korea. It contains pyranocoumarins, which are major active components including decursin ... + READ MORE
    Angelica gigas Nakai (AGN) is a traditional medicinal herb especially in Korea. It contains pyranocoumarins, which are major active components including decursin (D) and decursinol angelate (DA). This study was carried out to determine the change in active component content and antioxidant activity depending on the root diameter of AGN. Several processing steps are involved to use AGN roots as medicine. The dried AGN roots are divided into body (B), thick root (TkR), medium root (MR) and thin root (TnR) according to their diameter before cutting into medicine. The recovery rates of each root parts per 100 ㎏ were measured as 32.3±2.5, 9.0±1.0, 39.3±2.1 and 15.0±1.0%, respectively and the mean diameters were measured as 51.95±4.55, 7.05±0.89, 2.88±0.49 and 1.57±0.32 ㎜. Two index components, D and DA, were analyzed. The change of both D and DA content showed a similar tendency. Both D and DA content were increased as the root diameter decreased (higher in TnR). In addition, antioxidant activity was higher in B and TnR, and lower in TkR and MR than control. This study showed that the thinner the root diameter, the higher the D and DA content in AGN roots and that TnR has excellent antioxidant activity compared to other root parts, suggesting that the thinner root part of AGN could be used as a potential material. - COLLAPSE
    April 2019
  • Research Article

    Construction of Database System on Amylose and Protein Contents Distribution in Rice Germplasm Based on NIRS Data

    벼 유전자원의 아밀로스 및 단백질 성분 함량 분포에 관한 자원정보 구축

    Sejong Oh, Yu Mi Choi, Myung Chul Lee, Sukyeung Lee, Hyemyeong Yoon, Muhammad Rauf and Byungsoo Chae

    오세종, 최유미, 이명철, 이수경, 윤혜명, Muhammad Rauf, 채병수

    This study was carried out to build a database system for amylose and protein contents of rice germplasm based on NIRS (Near-Infrared ... + READ MORE
    This study was carried out to build a database system for amylose and protein contents of rice germplasm based on NIRS (Near-Infrared Reflectance Spectroscopy) analysis data. The average waxy type amylose contents was 8.7% in landrace, variety and weed type, whereas 10.3% in breeding line. In common rice, the average amylose contents was 22.3% for landrace, 22.7% for variety, 23.6% for weed type and 24.2% for breeding line. Waxy type resources comprised of 5% of the total germplasm collections, whereas low, intermediate and high amylose content resources share 5.5%, 20.5% and 69.0% of total germplasm collections, respectively. The average percent of protein contents was 8.2 for landrace, 8.0 for variety, and 7.9 for weed type and breeding line. The average Variability Index Value was 0.62 in waxy rice, 0.80 in common rice, and 0.51 in protein contents. The accession ratio in arbitrary ranges of landrace was 0.45 in amylose contents ranging from 6.4 to 8.7%, and 0.26 in protein ranging from 7.3 to 8.2%. In the variety, it was 0.32 in amylose ranging from 20.1 to 22.7%, and 0.51 in protein ranging from 6.1 to 8.3%. And also, weed type was 0.67 in amylose ranging from 6.6 to 9.7%, and 0.33 in protein ranging from 7.0 to 7.9%, whereas, in breeding line it was 0.47 in amylose ranging from 10.0 to 12.0%, and 0.26 in protein ranging from 7.0 to 7.9%. These results could be helpful to build database programming system for germplasm management. - COLLAPSE
    April 2019
  • Research Article

    The Disease Severity and Related Pathogens Caused by Root Rot on 6 Years Old Ginseng Cultivation Fields

    인삼 6 년근 수확지의 뿌리썩음병 발생현황 및 관련 병원균 동정

    Mun Won Seo, You Kyoung Han, Yeoung Seuk Bae and Seung Ho Lee

    서문원, 한유경, 배영석, 이승호

    Ginseng (Pnanx ginseng C. A. Meyer) is famous worldwide, and is very important cash crop and medicinal herb in Korea. It takes ... + READ MORE
    Ginseng (Pnanx ginseng C. A. Meyer) is famous worldwide, and is very important cash crop and medicinal herb in Korea. It takes four to five years to produce harvestable ginseng roots, and ginseng is attacked by several pathogens during cultivation. We investigated the disease rate caused by ginseng root rot from 6 years old ginseng cultivation fields (Chungnam; 9 fields, Chungbuk; 11 fields, Gangwon 5 fields). The highest disease severity was Dangjin D (2.9) and the lowest one was Gaesan C (0.6). Of the 625 isolations, 340 isolations were classified as Ilyonectria radicicola and Fusarium solani. Finally, genetic diversity of I. radicicola and F. solani was confirmed by sequence analysis. Among the I. radicicola group, I. mors-panacis, which is known as highly virulent pathogen, and I. liriodendri, I. robusta and I. cyclamicicola, which are weakly virulent pathogens, were identified. In the case of F. solani, it is divided into two groups, but it is necessary to conduct diversity research through genetic analysis and pathogenetic studies using various markers. Based on these results, it could be used as a basic data for control of ginseng root rot pathogens. - COLLAPSE
    April 2019
  • Research Article

    Effect of the Extracts from the Leaves and Branches of Sageretia thea on β-catenin Proteasomal Degradation in Human Colorectal and Lung Cancer Cells

    상동나무(Sageretia thea) 잎과 가지추출물의 대장암과 폐암세포의 β-catenin 분해 유도 활성

    Ha Na Kim, Gwang Hun Park, Jeong Dong Kim, Su Bin Park, Hyun Ji Eo and Jin Boo Jeong

    김하나, 박광훈, 김정동, 박수빈, 어현지, 정진부

    In this study, we evaluated the effect of branch (STB) and leave (STL) extracts from Sageretia thea on β-catenin level in human ... + READ MORE
    In this study, we evaluated the effect of branch (STB) and leave (STL) extracts from Sageretia thea on β-catenin level in human colorecal cancer cells, SW480 and lung cancer cells, A549. STB and STL dose-dependently suppressed the growth of SW480 and A549 cells. STB and STL decreased β-catenin level in both protein and mRNA level. MG132 decreased the downregulation of β-catenin protein level induced by STB and STL. However, the inhibition of GSK3β by LiCl or ROS scavenging by NAC did not block the reduction of β-catenin protein by STB and STL. Our results suggested that STB and STL may downregulate β-catenin protein level independent on GSK3β and ROS. Based on these findings, STB and STL may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer and lung cancer. - COLLAPSE
    April 2019
  • Research Article

    A Study on Growth Characteristics of Wild-simulated Ginseng (Panax ginseng C.A. Meyer) by Direct Seeding and Transplanting

    직파 및 이식재배 유형에 따른 산양삼의 생육특성 연구

    Kiyoon Kim, Daehui Jeong, Hyun-Jun Kim, Kwonseok Jeon, Mahnjo Kim and Yurry Um

    김기윤, 정대희, 김현준, 전권석, 김만조, 엄유리

    The this study was carried out to investigate the growth characteristics of wild-simulated ginseng by direct seeding and transplanting cultivation for develop ... + READ MORE
    The this study was carried out to investigate the growth characteristics of wild-simulated ginseng by direct seeding and transplanting cultivation for develop standard cultivation techniques of wild-simulated ginseng. Bonghwa experimental field were confirm to be suitable location environment for cultivation of wild-simulated ginseng. As a result of this study, the germination rate of wild-simulated ginseng was significantly highest when seed size was over 6.5 ㎜ in the spot seeding cultivation. In the case of transplanting, survival rate was significantly increased when the diameter of root was over 10 ㎜, planting distance was 7 ㎝, and the thickness of soil covering was less then 2 ㎝. The result of growth characteristics of wild-simulated ginseng by cultivation type, growth of stem in spot seeding cultivation was showed significantly increased when seed size over 6 ㎜, seeding number was 3 grains, and the seeding distance was less then 5 ㎝. Strip seeding cultivation was showed significantly increased in stem and root growth when seeding distance was 30 ㎝ grains and quantity of seeding was less then 23 g. In the case of transplanting cultivation, it’s was showed significantly increased in stem growth when diameter of root was over then 10 ㎜ and direction of rhizome was top and bottom. The results of this study was that to clearly establish the techniques of cultivation of managements and it’s will be suggest contribute to the industrial activation of wild-simulated ginseng. - COLLAPSE
    April 2019
  • Research Article

    Flora of Mt. Choejeong (Daegu)

    최정산(대구)의 관속식물상 연구

    Minji Jun, Eunmi Lee, Sunmi Park, Jongwu Bae, Myeongwu Na, Youjin Hwang, SuMi Choi and SeonJoo Park

    전민지, 이은미, 박선미, 배종우, 나명우, 황유진, 최수미, 박선주

    This study was carried out to investigate the vascular plants of Mt. Choejeong in Gachang-myeon, Daegu. From March 2017 to October 2018 ... + READ MORE
    This study was carried out to investigate the vascular plants of Mt. Choejeong in Gachang-myeon, Daegu. From March 2017 to October 2018, a total of 22 studies were conducted. The vascular plants surveyed were grouped into 560 taxa, including 104 families, 297 genera, 495 species, 4 subspecies, 51 varieties and 10 forma. Endemic plants 15 taxa, Rare plants 5 taxa, Red list plants 5 taxa, Floristic regional indicator plants 54 taxa, Naturalized plants 36 taxa were recorded. Among surveyed 560 taxa, edible, medicinal, ornamental, timber, pasturing, industrial and fiber plants included 246 taxa (29.2%), 228 taxa (27.1%), 164 taxa (19.5%), 61 taxa (7.2%), 13 taxa (1.5%), and 8 taxa (0.9%). And because people are coming and going more frequently than in the past, this will result in more frequent influx of naturalized plants and a threat to the habitat of the plants that are currently growing. - COLLAPSE
    April 2019
  • Research Article

    Unrecorded Alien Plant in South Korea: Ludwigia peploides subsp. montevidensis (Spreng.) P.H. Raven

    미기록 침입외래종: 꽃여뀌바늘

    Hye-Won Kim, Dong Chan Son, Soo Hyun Park, Chang-Seok Jang, Eun-Mi Sun, Hyeryun Jo, Seok Min Yun and Kae Sun Chang

    김혜원, 손동찬, 박수현, 장창석, 선은미, 조혜련, 윤석민, 장계선

    Alien invasive species are introduced with or without intent and spreading all over Korea. They are known to have negative effects on ... + READ MORE
    Alien invasive species are introduced with or without intent and spreading all over Korea. They are known to have negative effects on biodiversity such as economic and environmental damage and causing decrease or loss of native species. The habitats like wetland, reservoir and riverside are especially in danger of being invaded by alien species due to stress and disturbance. Therefore, Korea National Arboretum is steadily working on research and studies on managing alien invasive species. This research aims to collect basic information of Ludwigia peploides subsp. montevidensis (Spreng.) P.H. Raven which was found near riverside in Suwon-si and is concerned to become an invasive alien species. We expect the description, diagram and pictures of this taxon will be helpful for early detection and effective management. - COLLAPSE
    April 2019