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2018 Vol.31, Issue 4
August 2018. pp. 275-282
Paeoniae Radix has been used as a traditional medicine for various diseases including hepatic disease. However, the inhibitory effect of Paeoniae Radix on intestinal inflammation has not been fully understood yet. The aim of this study was to evaluate the effect of Paeoniae Radix on colitis induced by dextran sulfate sodium in mice. To investigate the protective effects of Paeoniae Radix, the colitis mice were induced by drinking water containing 5% dextran sulfate sodium for 7 days. Mice were randomized into groups receiving Paeoniae Radix (100 ㎎/㎏), sulfasalazine (150 ㎎/㎏) as a positive control, or water as a negative control. We evaluated the effects of Paeoniae Radix on clinical signs induced by dextran sulfate sodium, measuring weight loss, colon length, and disease activity index. Additionally, to find a possible explanation for the anti-inflammatory effects of Paeoniae Radix, we evaluated the effects of Paeoniae Radix on the interleukin-6 and cyclooxygenase-2 levels in colitis tissue. The results indicated that mice treated with dextran sulfate sodium showed measurable clinical signs, including weight loss and reduced colon length. However, Paeoniae Radix treatment significantly improved the weight loss and disease activity index as clinical symptoms. Moreover, Paeoniae Radix inhibited the interleukin-6 and cyclooxygenase-2 expression levels in colon tissues treated with dextran sulfate sodium. Taken together, the findings of this study suggest that Paeoniae Radix may be useful for treating intestinal inflammation, including ulcerative colitis.
  1. Agoff, S.N., T.A. Brentnall, D.A. Crispin, S.L. Taylor, S. Raaka, R.C. Haggitt, M.W. Reed, I.A. Afonina, P.S. Rabinovitch, A.C. Stevens, Z. Feng and M.P. Bronner. 2000. The role of cyclooxygenase-2 in ulcerative colitis-associated neoplasia. Am. J. Pathol. 157(3):737-745.10.1016/S0002-9440(10)64587-7
  2. Bae, K.H. 2003. Illustrated Book of Health for Longevity. Kyohaksa, Seoul, Korea. p. 426 (in Korean).
  3. Choi, C.H., Y.H. Kim, Y.S. Kim, B.D. Ye, K.M. Lee, B.I. Lee, S.A. Jung, W.H. Kim and H.Y. Lee. 2012. IBD study group of the Korean Association for the Study of Intestinal Diseases: Guidelines for the management of Ulcerative colitis. Korean J. Gastroenterol. 59(2):118-140.10.4166/kjg.2012.59.2.11822387836
  4. Chuang, W.C., W.C. Lin, S.J. Sheu, S.H. Chiou, H.C. Chang and Y.P. Chen. 1996. A comparative study on commercial samples of the roots of Paeonia vitchii and P. lactiflora. Planta. Med. 62(4):347-351.10.1055/s-2006-95789917252469
  5. Cooper, H.S., S.N. Murthy, R.S. Shah and D.J. Sedergran. 1993. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab. Invest. 69(2):238-249.8350599
  6. Danese, S., M. Sans and C. Fiocchi. 2004. Inflammatory bowel disease: the role of environmental factors. Autoimmun. Rev. 3(5):394-400.10.1016/j.autrev.2004.03.002
  7. Domènech, E. 2006. Inflammatory bowel disease: current therapeutic options. Digestion 73(1):67-76.10.1159/00008978116498254
  8. Hendel, J. and O.H. Nielsen. 1997. Expression of cyclooxygenase- 2 mRNA in active inflammatory bowel disease. Am. J. Gastroenterol. 92(7):1170-1173.9219792
  9. Hendrickson, B.A., R. Gokhale and J.H. Cho. 2002. Clinical aspects and pathophysiology of inflammatory bowel disease. Clin. Microbiol. Rev. 15(1):79-94.10.1128/CMR.15.1.79-94.200211781268 PMC118061
  10. Hsieh, C.L., C.Y. Cheng, T.H. Tsai, I.H. Lin, C.H. Liu, S.Y. Chiang, J.G. Lin, C.J. Lao and N.Y. Tang. 2006. Paeonol reduced cerebral infarction involving the superoxide anion and microglia activation in ischemia-reperfusion injured rats. J. Ethnopharmacol. 106(2):208-211.10.1016/j.jep.2005.12.02716458462
  11. Hyams, J.S. 2000. Inflammatory bowel disease. Pediatr. Rev. 21(9):291-295.10.1542/pir.21-9-29110970450
  12. Ishiguro, K., T. Ando, O. Maeda, M. Hasegawa, K. Kadomatsu, N. Ohmiya, Y. Niwa, R. Xavier and H. Goto. 2006. Paeonol attenuates TNBS-induced colitis by inhibiting NF-B and STAT1 transactivation. Toxicol. Appl. Pharmacol. 217(1): 35-42.10.1016/j.taap.2006.07.00216928387
  13. Ji, S.T., S.J. Lee, K.E. Lee, Y.T. Son and Y.K. Chung. 2002. Inhibitory effect of extracts from Paeoniae radixon Postprandial Hyperglycemia. J. Korean Soc. Food Sci. Nutr. 31(1):131-135. 10.3746/jkfn.2002.31.1.131
  14. Kawamori, T., C.V. Rao, K. Seibert and B.S. Reddy. 1998. Chemopreventive activity of elecoxib, a specific cyclooxygenase- 2 inhibitor, against colon carcinogenesis. Cancer Res. 58: 409-412.9458081
  15. Kim, J.H. and E.J. Kim. 2018. Evaluation of anti-oxidative, anti-thrombin, anti-invasive and pro-apoptotic activities of Paeonia japonica. Korean J. Plant Res. 31(1):16-23.
  16. Lee, B., Y.W. Shin, E.A. Bae, S.J. Han, J.S. Kim, S.S. Kang and D.H. Kim. 2008. Antiallergic effect of the root of Paeonia lactiflora and its constituents paeoniflorin and paeonol. Arch. Pharm. Res. 31(4):445-450. 10.1007/s12272-001-1177-618449501
  17. Li, Y., C. de Haar, M. Chen, J. Deuring, M.M. Gerrits, R. Smits, B. Xia, E.J. Kuipers and C.J. van der Woude. 2010. Disease- related expression of the IL-6/STAT3/SOCS3 signaling pathway in ulcerative colitis and ulcerative colitis-related carcinogenesis. Gut 59(2):227-235.10.1136/gut.2009.18417619926618
  18. Lichtenstein, G.R. and P. Rutgeerts. 2010. Importance of mucosal healing in ulcerative colitis. Inflamm. Bowel. Dis. 16(2):338-346. 10.1002/ibd.2099719637362
  19. Lim, K.S., J.K. Park, M.H. Jeong, I.H. Bae, D.S. Park, J.W. Shim, J.H. Kim, H.K. Kim, S.S. Kim, D.S. Sim, Y.J. Hong, J.H. Kim and Y. Ahn. 2018. Anti-inflammatory effect of gallic acid-eluting stent in a Porcine Coronary Restenosis Model. Acta. Cardiol. Sin. 34(3):224-232.
  20. Lyu, Z.K., C.L. Li, Y. Jin, Y.Z. Liu, X. Zhang, F. Zhang, L.N. Ning, E.S. Liang, M. Ma, W. Gao, M.X. Zhang and D.S. Liu. 2017. Paeonol exerts potential activities to inhibit the growth, migration and invasion of human gastric cancer BGC823 cells via downregulating MMP 2 and MMP 9. Mol. Med. Rep. 16(5):7513-7519.10.3892/mmr.2017.7576
  21. Matuk, R., J. Crawford, M.T. Abreu, S.R. Targan, E.A. Vasiliauskas and K.A. Papadakis. 2004. The spectrum of gastrointestinal toxicity and effect on disease activity of selective cyclooxygenase-2 inhibitors in patients with inflammatory bowel disease. Inflamm. Bowel Dis. 10(4):352-356.10.1097/00054725-200407000-00005
  22. Ministry of Food and Drug Safety. 2014. Korean Pharmacopeia (tenth edition) Monographs Part2. Ministry of Food and Drug Safety, Cheongju, Korea. p. 1341.
  23. Murthy, S.N., H.S. Cooper, H. Shim, R.S. Shah, S.A. Ibrahim and D.J. Sedergran. 1993. Treatment of dextran sulfate sodium-induced murine colitis by intracoloniccyclosporin. Dig. Dis. Sci. 38(9):1722-1734.10.1007/BF013031848359087
  24. Ngan, L.T., M.J. Jang, M.J. Kwon and Y.J. Ahn. 2015. Antiviral activity and possible mechanism of action of constituents identified in Paeonia lactiflora root toward human rhinoviruses. PLoS One 10(4):e0121629. 10.1371/journal.pone.012162925860871PMC4393083
  25. Ogata, H. and T. Hibi. 2003. Cytokine and anti-cytokine therapies for inflammatory bowel disease. Curr. Pharm. Des. 9(14): 1107-1113.10.2174/138161203345503512769750
  26. Oriental Medicine Pharmacology Textbook Compilation Committee. 2010. Oriental Medicine Pharmacology (third edition). Shinilbooks, Seoul, Korea. pp. 694-700 (in Korean).
  27. Papadakis, K.A. and S.R. Targan. 2000. Role of cytokines in the pathogenesis of inflammatory bowel disease. Annu. Rev. Med. 51:289-298.10.1146/
  28. Park, K.K., H.S. Park, Y.H. Cho, H.O. Yang, W.K. Ko, H.S. Ro and Y.E. Lee. 1999. Isolation of hyperlipidemic substancesfrom methanol extract of Paeoniae radix. J. Pharm. Investig. 29: 55-60.
  29. Ro, H.S., W.K. Ko, H.O. Yang, K.K. Park, Y.H. Cho and H.S. Park. 1997. Effect of several solvent extracts from Paeoniae radix on experimental hyperlipidemia in rats. J. Pharm. Investig. 27:145-151.
  30. Roberts, P.J., K. Morgan, R. Miller, J.O. Hunter and S.J. Middleton. 2001. Neuronal COX-2 expression in human myenteric plexus in active inflammatory bowel disease. Gut 48(4):468-472. 10.1136/gut.48.4.46811247889PMC1728255
  31. Rogler, G. and T. Andus. 1998. Cytokines in inflammatory bowel disease. World J. Surg. 22(4):382-389. 10.1007/s0026899004019523521
  32. Tsai, H.Y., H.Y. Lin, Y.C. Fong, J.B. Wu, Y.F. Chen, M. Tsuzuki and C.H. Tang. 2008. Paeonol inhibits RANKL- induced osteoclastogenesis by inhibiting ERK, p38 and NF-kappaB pathway. Eur. J. Pharmacol. 588(1):124-133. 10.1016/j.ejphar.2008.04.02418495114
  33. Vázquez Cisneros, L.C., P. López-Uriarte, A. López-Espinoza, M. Navarro Meza, A.C. Espinoza-Gallardo and M.B. Guzmán Aburto. 2017. Effects of green tea and its epigallocatechin (EGCG) content on body weight and fat mass in humans: a systematic review. Nutr. Hosp. 34(3):731-737.10.20960/nh.75328627214
  34. Wu, H.K. and S.J. Sheu. 1996. Capillary electrophoretic determination of the constituents of Paeoniae radix. J. Chromatogr. A. 753(1):139-146.10.1016/S0021-9673(96)00525-0
  35. Zhang, J., W. Dou, E. Zhang, A. Sun, L. Ding, X. Wei, G. Chou, S. Mani and Z. Wang. 2014. Paeoniflorin abrogates DSS- induced colitis via a TLR4-dependent pathway. Am. J. Physiol. Gastrointest. Liver Physiol. 306(1):G27-36.10.1152/ajpgi.00465.201224232001PMC3920084
  36. Zhong, S.Z., Q.H. Ge, R. Qu, Q. Li and S.P. Ma. 2009. Paeonol attenuates neurotoxicity and ameliorates cognitive impairment induced by d-galactose in ICR mice. J. Neurol. Sci. 277(1-2): 58-64.10.1016/j.jns.2008.10.00819007942