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2019 Vol.32, Issue 4 Preview Page

Research Article

Development of SNP Molecular Marker for Red-fleshed Color Identification of Peach Genetic Resources

복숭아 유전자원의 적색 과육 판별 SNP 분자표지 개발

Se Hee Kim1*
,
Eun Young Nam1
,
Kang Hee Cho1
,
Ji Hae Jun2
,
Kyeong Ho Chung2
김 세희1*
,
남 은영1
,
조 강희1
,
전 지혜2
,
정 경호2
1Researcher, Fruit Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, Korea
2Senior Rsearcher, Fruit Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, Korea
1농촌진흥청 국립원예특작과학원 과수과, 연구사
2농촌진흥청 국립원예특작과학원 과수과, 연구관
* Corresponding Author
31 August 2019. pp. 303-311
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Abstract

Various colors of fruit skin and flesh are the most popular commercial criteria for peach classification. In order to breed new red-fleshed peach cultivar, many cross seedlings and generations should be maintained. Therefore it is necessary to develop early selection markers to screen seedlings with target traits to increase breeding efficiency. For the comparison of transcription profiles in peach cultivars differing in flesh color expression, two cDNA libraries were constructed. Differences in gene expression between red-fleshed peach cultivar, ‘Josanghyeoldo’ and white-fleshed peach cultivar, ‘Mibaekdo’ were analyzed by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the two cultivars were selected for nucleotide sequence determination and homology searches. Putative single nucleotide polymorphisms (SNP) were screened from peach EST contigs by high resolution melting (HRM) analysis displayed specific difference between 8 red-fleshed peach cultivars and 24 white-fleshed peach cultivars. All 72 pairs of SNPs were discriminated and the HRM profiles of amplicons were established. In the study reported here, the development of SNP markers for distinguishing between red and white fleshed peach cultivars by HRM analysis offers the opportunity to use DNA markers. This SNP marker could be useful for peach marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in peach cultivars.

Keywords
Anthocyanin
Breeding
Cultivars
Gene expression
High resolution melting
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :4
  • Pages :303-311
  • Received Date : 2019-04-14
  • Revised Date : 2019-06-04
  • Accepted Date : 2019-06-10
  • DOI :https://doi.org/10.7732/kjpr.2019.32.4.303
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