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2023 Vol.36, Issue 6 Preview Page

Research Article

Efficient Cryopreservation of in vitro Grown Shoot Tips of Pear (Pyrus spp.) by Droplet-vitrification
Jae-Young Song1
,
Jinjoo Bae3
,
Ji-Won Han2*
,
Ho Cheol Ko2
,
Ho-sun Lee1
,
Sung-Hee Nam1
,
Jung-Ro Lee1
,
Byeong Hyeon Yun4
,
Keumsun Kim5
,
Kyungho Won6
,
Il Sheob Shin7
1Post-doc, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
2Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
3Senior Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
4Researcher, Fruit Research Division, National Institute of Horticultural & Herbal Science, Wanju 55365, Korea
5Post-doc, Pear Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA), Naju 58216, Korea
6Researcher, Pear Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA), Naju 58216, Korea
7Senior Researcher, Pear Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA), Naju 58216, Korea
*Corresponding Author
1 December 2023. pp. 571-579
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Abstract

In this study, cryopreservation by droplet-vitrification was applied to pear (Pyrus spp.) germplasm. We focused on the development and assessment of various strategies for the selection of suitable tissue, osmoprotection, and dehydration. We also evaluated post-thaw recovery of cryopreserved explants by droplet-vitrification. Preferentially, we tested the effects of preculture and loading treatments to determine which tissues were more suitable, either the apical shoot tips or the axillary buds. Apical shoot tips showed the better regrowth rate than in vitro axillary buds. The most effective techniques for cryopreservation were as follows. Shoots from in vitro seedlings which had been cultured for about 5-6 weeks were cold-hardened at 4℃ for one week, excised shoot tips were precultured on liquid MS medium including 0.3 M sucrose for 31 hours and 0.7 M sucrose for 17 hours, osmoprotected in loading solution (LS) for 40 min, and then cryoprotected in dehydration solution (PVS3) for 90 min. In addition, we found that regrowth rates of explants on regrowth medium after exposure to liquid nitrogen (LN) were higher than those on MS medium. Results indicated that the highest regrowth percentage was 95.6% for ‘Bartlett’ cultivar and 68.9% for ‘BaeYun No.3’ cultivar. Consequently, apical shoot tips of two pear cultivars, ‘Bartlett’ (P. communis) and ‘BaeYun No.3’ (P. pyrifolia), were successfully cryopreserved by droplet-vitrification. Results of this study show that the enhanced droplet-vitrification method described in the present study could be used as an effective means for long-term storage of pear genetic resources.

Keywords
Cryopreservation
Droplet-vitrification
Pyrus
Regrowth
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :6
  • Pages :571-579
  • Received Date : 2023-10-09
  • Revised Date : 2023-11-10
  • Accepted Date : 2023-11-14
  • DOI :https://doi.org/10.7732/kjpr.2023.36.6.571
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