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2020 Vol.33, Issue 4 Preview Page

Research Article

Induction of Apoptosis by Ethanol Extract of Lythrum anceps (Koehne) Makino in Human Leukemia U937 Cells

인체백혈병 U937 세포에서 부처꽃 에탄올추출물에 의한 apoptosis 유도

Jin-Woo Jeong1†
,
Chul Hwan Kim1†
,
Young-Kyung Lee1
,
Yong Hwang1
,
Ki Won Lee2
,
Kyung-Min Choi1
,
Jung Il Kim3*
정 진우1†
,
김 철환1†
,
이 영경1
,
황 용1
,
이 기원2
,
최 경민1
,
김 정일3*
1Researcher, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea
2Researcher, Department of Occupational and Environmental Medicine, Dong-a University, Busan 49201, Korea
3Professor, Department of Occupational and Environmental Medicine, Dong-a University, Busan 49201, Korea
1국립낙동강생물자원관, 연구원
2동아대학교 의과대학 직업환경의학교실, 연구원
3동아대학교 의과대학 직업환경의학교실, 교수
* Corresponding Author
† These authors contributed equally to this work.
1 August 2020. pp. 279-286
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Abstract

Purple loosestrife-Lythrum anceps (Koehne) Makino is a herbaceous perennial plant belonging to the Lythraceae family. It has been used for centuries in Korea and other Asian traditional medicine. It has been showed pharmacological effects, including anti-oxidant and anti-microbial effects. However, the mechanisms underlying its anti-cancer effect are not yet understood. In this study, we investigated the mechanism of apoptosis signaling pathways by ethanol extract of Lythrum anceps (Koehne) Makino (ELM) in human leukemia U937 cells. Treatment with ELM significantly inhibited cell growth in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies (ApoBDs), DNA fragmentation and increased populations of sub-G1 ratio. Induction of apoptosis by ELM was connected with up-regulation of death receptor (DR) 4 and DR5, pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic Bcl-2 protein, and inhibitor of apoptosis protein (IAP) family proteins, depending on dosage. This induction was associated with Bid truncation, mitochondrial dysfunction, proteolytic activation of caspases (-3, -8 and –9) and cleavage of poly(ADP-ribose) polymerase protein. Therefore, our data indicate that ELM suppresses U937 cell growth by activating the intrinsic and extrinsic apoptosis pathways, and thus may have applications as a potential source for an anti-leukemic chemotherapeutic agent.

Keywords
Apoptosis
Caspase
Leukemia U937 cells
Lythrum anceps (Koehne) Makino
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :4
  • Pages :279-286
  • Received Date : 2020-03-31
  • Revised Date : 2020-07-19
  • Accepted Date : 2020-07-22
  • DOI :https://doi.org/10.7732/kjpr.2020.33.4.279
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