Hibiscus manihot leaves Attenuate Accumulation of Lipid Droplets by Activating Lipolysis,  Browning and Autophagy,  and Inhibiting Proliferation of 3T3-L1 Cells

Na Gyeong Geum; Jeong Won Choi; Hyeok Jin Choi; Gwang Hyeon Ryu; Jin Boo Jeong

doi:10.7732/kjpr.2023.36.6.541

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2023 Vol.36, Issue 6 Next Page

Research Article

Hibiscus manihot leaves Attenuate Accumulation of Lipid Droplets by Activating Lipolysis, Browning and Autophagy, and Inhibiting Proliferation of 3T3-L1 Cells

1 December 2023. pp. 541-548

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Abstract

In the present study, the effects of HML on lipolysis, adipocyte browning, autophagy, and proliferation were investigated. HML affected lipolysis by increasing the protein levels of ATGL and HSL, and phosphorylation levels of HSL and AMPK. Furthermore, HSL decreased the perilipin-1 levels. In addition, free glycerol content was increased by HML treatment. HML affected adipocyte browning by increasing the protein levels of UCP-1, PGC-1α, and PRDM16. In addition, HML affected autophagy by increasing the levels of LC3-I and LC3-II, and decreasing those of SQSTM1/p62. Moreover, HML affected adipocyte proliferation by suppressing the proliferation of 3T3-L1 cells due to arrest of the cell cycle via blocking the expression of β-catenin and cyclin D1. These results suggest that HML induces lipolysis, adipocyte browning, autophagy, and inhibits excessive proliferation of adipocytes.

Keywords

Anti-obesity activity

Hibiscus manihot

Adipocytes

Lipolysis

Adipocyte browning

Autophagy

Adipocyte proliferation

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Information

Publisher :The Plant Resources Society of Korea
Publisher(Ko) :한국자원식물학회
Journal Title :Korean Journal of Plant Resources
Journal Title(Ko) :한국자원식물학회지
Volume : 36
No :6
Pages :541-548
Received Date : 2023-10-16
Accepted Date : 2023-10-30
DOI :https://doi.org/10.7732/kjpr.2023.36.6.541

Korean Journal of Plant Resources 한국자원식물학회지 ISSN:1226-3591(Print) 2287-8203(Online)

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