All Issue

2018 Vol.31, Issue 4 Preview Page
August 2018. pp. 294-302
Abstract

This study was conducted to compare anti-inflammatory effect of Robinia pseudoacacia L. using different extraction methods (water extraction, ethanol extraction and high temperature extraction). We investigated anti-inflammatory effect of Robinia pseudoacacia L. extract (RP1, water extract; RP2, ethanol extract; RP3, high temperature extract) on lipopolysaccharide (LPS)-stimulated inflammation using Raw 264.7 cell. Cells were treated with various concentrations (12.5, 25, 50, 100 or 200 ㎍/㎖) of water extract, ethanol extract and high temperature extract. Cytotoxicity was not observed on Raw 264.7 cells, LPS-stimulated production of NO (nitric oxide), PGE2 (prostaglandin E2) and cytokines (TNF-α, IL-6 and IL-1β) was reduced by RP3 treatment more than RP1 and RP2. In conclusion, these results indicated that inflammation on Raw 264.7 cells was improved by RP3. Treatment of RP3 could be used to natural medicine for improving inflammatory response. However, further experiment is required to observe how the high temperature extraction at 500℃ for 48 h influences on alteration of active ingredient in Robinia pseudoacacia L., and conducts the inflammation signal pathway on Raw 264.7 cells.

References
  1. Ahn, S.H., D.G. Jeong, S.J. Oh, S.H. Lee and D.H. Chung. 2017. GM-CSF and IL-4 produced by NKT cells inversely regulate IL-1β production by macrophages. Immunol Lett. 82:50-56.10.1016/j.imlet.2017.01.00328063891
  2. Blaise, G.A., D. Gauvin, M. Gangal and S. Authier. 2005. Nitric oxide, cell signaling and cell death. Toxicology 15:177-192.10.1016/j.tox.2004.11.03215691583
  3. Bradley, J.R. 2008. TNF-mediated inflammatory disease. J Pathol. 214:149-160.10.1002/path.228718161752
  4. Borthwick, L.A. 2016. The IL-1 cytokine family and its role in inflammation and fibrosis in the lung. Semin Immunopathol. 38:517-534.10.1007/s00281-016-0559-z27001429 PMC4896974
  5. Chung, H.Y., M. Cesari, S. Anton, E. Marzetti, S. Giovannini, A.Y. Seo, C. Carter, B.P. Yu and C. Leeuwenburgh. 2009. Molecular inflammation: underpinnings of aging and age- related disease. Ageing Res Rev. 8:18-30.10.1016/j.arr.2008.07.00218692159PMC3782993
  6. Choi, Y.J., W.S. Jo, H.J. Kim, B.H. Nam, E.Y. Kang, S.J. Oh, G.A. Lee and M.H. Jeong. 2010. Anti-inflammatory effect of Chlorella ellipsoidea extracted from seawater by organic solvents. Korean J Fish Aquat Sci. 43:39-45.10.5657/kfas.2010.43.1.039
  7. Emanuela, R. and A.F. Garret. 2011. Prostaglandins and inflammation. Arterioscler Thromb Vasc Biol. 31:986-1000.10.1161/ATVBAHA.110.20744921508345PMC3081099
  8. Guo, H.F. and M.H. Wang. 2018. Anti-inflammatory and anti- cancer effect of Stachys affinis Tubers. Korean J Plant Res. 30:679-685.
  9. Higuchi, M., N. Higashi, H. Taki and T. Osawa. 1990. Cytolytic mechanism of activated macrophages. tumor necrosis factor and L-arginine-dependent mechanism act as synergistically as the major cytolytic mechanism of activated macrophage. J Immunol. 144:1425-1431.2303713
  10. Jaffrey, S.R. and S.H. Snyder. 1995. Nitric oxide: a neural messenger. Annu Rev Cell Dev Biol. 11:417-440.10.1146/annurev.cb.11.110195.0022218689564
  11. Ji, J.D., Y.H. Lee and G.G. Song. 2004. Prostaglandin E2 (PGE2): roles in immun responses and inflammation. J Korean Rheum Assoc. 11:307-315.
  12. Kwon, J.H., M.W. Byun and Y.H. Kim. 1995. Chemical composition of acacia flower (Robinia pseudo-acacia). Korean J Food Sic Technol. 27:789-793.
  13. Kim, Y.K. and S.K. Kim. 2002. Nitric oxide and dental pulp. J Korean Acad Oper Dent. 27:543-551.10.5395/JKACD.2002.27.5.543
  14. Kwon, Y.J., K.H. Kim and H.K. Kim. 2002. Changes of total polyphenol content and antioxidant activity of Ligularia fischeri extracts with different microwave-assisted extraction condritions. Kor J Food Preserv. 9:332-337.
  15. Kim, H.N., S.B. Park, G.H. Park, H.J. Eo, J.H. Song, H.Y. Kwon and J.B. Jeong. 2018. Anti-inflammatory effect of the root extracts from Hibiscus syriacus in LPS-stimulated RAW 264.7 cells. Korean J Plant Res. 31:211-217.
  16. Lowenstein, C.J., E.W. Alley, P. Raval, A.M. Snowman, S.H. Synder, S.W. Russcll and W.J. Murphy. 1993. Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide. PNASU. 90:9730-9734.10.1073/pnas.90.20.9730
  17. Lee, S.H., S.J. Suh, K.H. Lee, J.B. Yang, S.U. Choi and S.S. Park. 2013. Anti-inflammatory effect of Peel extracts from Citrus fruits. J Fd Hyg Safety 28:342-348.10.13103/JFHS.2013.28.4.342
  18. Lee, J.W. and Y.J. Kang. 2018. Anti-inflammatory effect of Abeliophyllum distichum Flower extract and associated MAPKs and NF-κB pathway in Raw264.7 cells. Korean J Plant Res. 31:202-210.
  19. Medzhitov, R. 2008. Origin and physiological roles of inflammation. Nature 454:428-435.10.1038/nature0720118650913
  20. Park, J.H., H.S. Lee, H.C. Mun, D.H. Kim, N.S. Seong, H.G. Jung, J.K. Bang and H.Y. Lee. 2004. Improvement of anticancer activation of ultrasonificated extracts from Acanthopanax senticosus Harms, Ephedra sinica Stapf, Rubus coreanus Miq. and Artemisia capillaris Thunb. Kor J Med Crop Sci. 12: 273-278.
  21. Popa, C., M.G. van, Netea, P.L. Riel, J.W. van, der Meer and A.F. Stalenhoef. 2007. The role of TNF-alpha in chronic inflammatory condritions, intermediary metabolism, and cardiovascular risk. J Lipid Res. 48:751-762.10.1194/jlr.R600021-JLR20017202130
  22. Pelegrin, P., C. Gutierrez and A. Surprenant. 2008. P2X7 receptor differentially couples to distinct release pathways for IL-1beta in mouse macrophage. J Immunol. 180:7147-7157.10.4049/jimmunol.180.11.714718490713
  23. Park, H.M. and J.H. Hong. 2014. Effect of extraction methods on antioxidant activities of Mori ramulus. J Korean Soc Food Sci Nutr. 43:1709-1715.10.3746/jkfn.2014.43.11.1709
  24. Park, S.B., H.M. Song, H.N. Kim, G.H. Park, H.J. Son, Y. Um, J.A. Park and J.B. Jeong. 2018. Anti-inflammatory effect of Biji (Soybean curd residue) on LPS-stimulated RAW264.7 cells. Korean J Plant Res. 31:117-123.
  25. Patra, J.K., E.S. Kim, K. Oh, H.J. Kim, R. Dgakal, Y Kim, and K.H. Baek, 2015. Bactericidal effect of extracts and metabolites of Robinia pseudoacacia L. on streptococcus mutans and porphyromonas gingivalis causing dental plaque and periodontal inflammatory diseases. Molecules 8:6128-6139.10.3390/molecules2004612825856062
  26. Ren, K. and R. Torres. 2009. Role of inerleukin-1beta during pain and inflammation. Brain Res Rev. 60:57-64.10.1016/j.brainresrev.2008.12.02019166877PMC3076185
  27. Shin, Y.H., H.J. Kim, J.Y. Lee, Y.J. Cho and B.J. An. 2012. Major compound analysis and assessment of natural essential oil on anti-oxidative and anti-microbial effects. J Life Sci. 22(10):1344-1351. 10.5352/JLS.2012.22.10.1344
  28. Woo, J.H., S.L. Shin, Y.D. Chang and C.H. Lee. 2010. Antioxidant effect according to extraction method in extracts of Dendranthema zawadskii var. yezoense and Cosmos bipinnatus. Kor J Hort Sci Technol. 28:462-468.
  29. Youn, S.H., J.S. Han and A.J. Kim. 2017. Anti-oxidant and anti-bacterial aactivities of mouthwash prepared with acacia flower, songin, and topan solar salt. Asian J Beauty Cosmetol. 15:160-168.10.20402/ajbc.2016.0119
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :4
  • Pages :294-302
  • Received Date :2018. 04. 05
  • Accepted Date : 2018. 07. 14