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2020 Vol.33, Issue 6 Preview Page

Research Article

Preconditioning for Cryopreservation of in vitro Grown Bulblets of Lily using Droplet-Vitrification
Jae-young Song1
,
Young-yi Lee2†
,
Jung-yoon Yi2
,
Jung-ro Lee2
,
Mun-sup Yoon3
1Post-doc, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
2Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
3Senior Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
*youngyi@korea.kr
1 December 2020. pp. 689-695
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Abstract

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. ‘Milky way’. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

Keywords
Bulb
Cryopreservation
Droplet-vitrification
Lilium
Regrowth
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :6
  • Pages :689-695
  • Received Date : 2020-11-06
  • Revised Date : 2020-11-24
  • Accepted Date : 2020-11-24
  • DOI :https://doi.org/10.7732/kjpr.2020.33.6.689
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