• Research Article

    Effects of Cultivated Wild Panax ginseng Extract on the Proliferation, Differentiation and Mineralization of Osteoblastic MC3T3-E1 Cells

    산양삼(cultivated wild Panax ginseng) 추출물이 조골세포 활성에 미치는 영향

    Hyun-Ju Seo, Hyun Ji Eo, Hyun Jun Kim, Kwon Seok Jeon, Gwang Hun Park, Se Chul Hong, Jin Boo Jeong

    서현주, 어현지, 김현준, 전권석, 박광훈, 홍세철, 정진부

    Panax ginseng C.A. Meyer (P. ginseng) is known to exert a wide range of pharmacological effects both in vitro and in ... + READ MORE
    Panax ginseng C.A. Meyer (P. ginseng) is known to exert a wide range of pharmacological effects both in vitro and in vivo. Although studies on ginsenoside, antioxidant activity, and anticancer effect of the cultivated wild Panax ginseng (CWP) have been conducted, there is little research on the effect of CWP extract on bone metabolism. In this study, we investigated the potential anti-osteoporotic properties of CWP on the growth and differentiation of MC3T3-E1 cells. CWP significantly increased the viability and proliferation of MC3T3-E1 cells. CWP activated intracellular alkaline phosphatase (ALP) activity in MC3T3-E1 cells. In addition, CWP increased the mineralized nodules in MC3T3-E1 cells. Furthermore, CWP increased the expression of genes such as Runx2, ALP, OPN and OCN associated with osteoblast growth and differentiation in a dose-dependent manner. - COLLAPSE
    August 2020
  • Research Article

    Identification and Characterization of Fibrinolytic Compound from Cornus officinalis S. et Z

    산수유(Cornus officinalis)로부터 혈전용해물질의 확인 및 특성 연구

    Jun-Ho Kim

    김준호

    The objective of this study was to identify and characterize fibrinolytic compound from Cornus officinalis. Cornus officinalis. Hot water extract was fractionated ... + READ MORE
    The objective of this study was to identify and characterize fibrinolytic compound from Cornus officinalis. Cornus officinalis. Hot water extract was fractionated into hexane, chloroform, ethyl acetate, butanol, and water fractions. Assays for fibrinolytic activity indicated that only the ethyl acetate fraction had significant efficacy at 1.36 plasmin units/mL. Isolation of fibrinolytic compound was carried out on Amberlite IRA-400, Sephadex LH-20 and active charcoal column chromatography. HPLC analysis of the purified fibrinolytic compound showed retention time (RT) same as authentic malic acid. LC / MS / MS in negative mode showed the same peak at m/z 133, confirming that the purified compound was malic acid with a molecular weight 134 Da. The compound showed fibrinolytic activity of 0.69 plasmin units/mL, 14.62% of thrombin inhibitory activity, 6.42% of antioxidative activity, and 17.28% of α-glucosidase inhibitory activity. The purified compound hydrolyzed γ subunits of human fibrinogen. In conclusion, malic acid isolated from Cornus officinalis might have potential to be developed as ingredient for biofunctional foods to prevent cardiovascular diseases. - COLLAPSE
    August 2020
  • Research Article

    Comparative Study of Antioxidant Activities at Different Cultivation Area and Harvest Date of the Gomchwi ‘Sammany’ Variety

    곰취 ‘쌈마니’ 품종의 재배지역 및 수확기별 항산화 활성 비교

    Jong Taek Suh, Ki Deog Kim, Hwang Bae Sohn, Su Jeong Kim, Su Young Hong and Yul Ho Kim

    서종택, 김기덕, 손황배, 김수정, 홍수영, 김율호

    This study was conducted for the comparison of antioxidant activities in different cultivation areas, harvest date and processing statuses in the new ... + READ MORE
    This study was conducted for the comparison of antioxidant activities in different cultivation areas, harvest date and processing statuses in the new variety ‘Sammany’ of Gomchwi (Fischer ligularia). The lowland was Gangneung, 20 m above sea level, and the highland was Daegwallyeong, 750 m above sea level. The total phenolic contents, flavonoids, DPPH free radical scavenging activities, and reducing power were analyzed by freezing and drying the raw and blanched of Gomchwi leaves from April 18th to July 15th, and extracting them through in the use of methanol. Total phenolic contents, flavonoids, DPPH free radical scavenging activities and reducing power were found to be more pronounced during May than they were in the period of early harvest on April 18th in the lowland, as well as to be more effective during June than in May in the highland. Total phenolic contents, flavonoids, DPPH free radical scavenging activities, and reducing power by region, harvest date, and processing status were more effective in May than the early harvest of April in flat areas, and antioxidant activity in the highlands was elevated as of June. When compared with raw and blench leaves, raw leaves exhibited higher antioxidant activities across the board. Highland cultivation also displayed higher antioxidant activities than did lowland cultivation. Combining the above results, the ‘Sammany’ variety of Gomchwi was found to be more active in terms of antioxidants, which were harvested in May in flat areas and June in the highlands. Also, consuming them raw showed higher antioxidant activity than when blanched. - COLLAPSE
    August 2020
  • Research Article

    Study on the Correlation between the Ginsenoside Contents and Growth Characteristics of Wild-simulated Ginseng with Different Year-Roots (Panax ginseng C.A. Meyer)

    산양삼 연근별 생육특성과 진세노사이드 함량 간의 상관관계 연구

    Kiyoon Kim, Yurry Um, Hyun-Ji Eo, Hong Woo Park, Kwon Seok Jeon, Hyun-Jun Kim

    김기윤, 엄유리, 어현지, 박홍우, 전권석, 김현준

    The aim of this study was to investigate the correlation between growth characteristics and ginsenoside contents of 7 and 13-year-old wild-simulated ginseng ... + READ MORE
    The aim of this study was to investigate the correlation between growth characteristics and ginsenoside contents of 7 and 13-year-old wild-simulated ginseng. The results of growth characteristics such as rhizome length, root length, fresh weight, cross-section area, surface area and volume were shows significantly higher in 13-year-old wild-simulated ginseng compare to 7-year-old wild-simulated ginseng. In the case of 11 ginsenoside contents, the contents of G-Rb1, Rb2, Rc, Rd, Re, Rf, Rg1 and Rg2 were shows significantly higher in 13-year-old wild-simulated ginseng compare to 7-year-old wild-simulated ginseng. In addition, in the comparative analysis of ginsenoside contents between wild-simulated ginseng and cultivated ginseng, 13-year-old wild-simulated ginseng was shows significantly higher G-Rb1, Rd, Re, Rf and Rg1 ginsenoside contents compare to 4-year-old and 5-year-old cultivated ginseng. In the result of correlation analysis between growth characteristics and ginsenoside contents, the G-Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2 ginsenoside was shows significantly positive correlation with rhizome length, fresh weight, cross-section area, surface area, volume, while as the contents of G-Rb1, Re, Rf, Rg2 was shows significantly negative correlation with shoot diameter. The results of this study was might be help to provide useful information on the establish quality standard by the investigate correlation analysis between growth characteristics and ginsenoside content of wild-simulated ginseng. - COLLAPSE
    August 2020
  • Research Article

    Inhibitory Activity of Lonicera caerulea Against Cell Proliferation in Human Colorectal Cancer Cells

    댕댕이나무(Lonicera caerulea)의 대장암세포 생육억제 활성

    Mi-Yun An, Hyun Ji Eo, Ho-Jun Son, Gwang Hun Park, Jin Boo Jeong

    안미연, 어현지, 손호준, 박광훈, 정진부

    In this study, we evaluated the effect of the extracts from Lonicera caerulea leaves (LCLE), branches (LCBE) and fruits (LCFE) on the ... + READ MORE
    In this study, we evaluated the effect of the extracts from Lonicera caerulea leaves (LCLE), branches (LCBE) and fruits (LCFE) on the cell growth and migration in human colorectal cancer cells, HCT116 and SW480 cells. LCLE and LCBE dose- and time-dependently inhibited the proliferation of HCT116 and SW480 cells. However, LCFE did not affect the proliferation of HCT116 and SW480 cells. In addition, LCLE and LCBE dramatically cell migration and wound healing in HCT116 cells. LCLE and LCBE decreased β-catenin protein level but not mRNA level in HCT116 and SW480 cells. Furthermore, LCLE decreased TCF4 level in both protein and mRNA level in HCT116 and SW480 cells. However, LCBE decreased TCF4 protein level but not mRNA level in HCT116 and SW480 cells. Based on these findings, LCLE and LCBE may inhibit the cell proliferation and migration through blocking Wnt signaling activation in human colorectal cancer cells. Therefore, LCLE and LCBE may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer. - COLLAPSE
    August 2020
  • Research Article

    Berchemia floribunda-mediated Proteasomal Degradation of CyclinD1 via GKS3β-dependent Threonine-286 Phosphorylation in Human Colorectal Cancer Cells

    인간 대장암 세포에 대한 먹넌출 추출물의 GSK3β 의존성 threonine-286 인산화를 통한 Cyclin D1 분해

    Yeongyeong Kang, Hyun Ji Eo, Da Som Kim, Youngki Park, Jeong Ho Song, Gwang Hun Park

    강연경, 어현지, 김다솜, 박영기, 송정호, 박광훈

    In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of the Berchemia floribunda (BF) which ... + READ MORE
    In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of the Berchemia floribunda (BF) which belongs to Rhamnaceae against human colorectal cancer cells. The treatment of BF decreased the cell proliferation in HCT116 cell and suppressed cellular accumulation of Cyclin D1 protein. Inhibition of proteasomal activity by MG132 attenuated BF-mediated Cyclin D1 downregulation and Cyclin D1 was decreased in the cell treated with BF. These findings indicates that BF-mediated Cyclin D1 downregulation may be result from Cyclin D1 proteasomal degradation. Additionally, BF-mediated Cyclin D1 degradation was blocked in the presence of LiCl, a GSK3β inhibitor, but not PD98059, SP600125, SB203580, Bay11-7082, LY294002 an ERK1/2 inhibitor, JNK inhibitor, p38 inhibitor, IκK inhibitor and PI3K inhibitor. Furthermore, BF phosphorylated Cyclin D1 at threonine-286 (Thr286), and LiCl-induced GSK3β inhibition reduced the BF mediated phosphorylation of Cyclin D1 at Thr286. These results suggested that BF may downregulate Cyclin D1 expression as a potential anti-cancer target through GSK3β dependent Cyclin D1 degradation. Therefore, this study provides that the extract of BF has anticancer activity against human colorectal cancer cells. - COLLAPSE
    August 2020
  • Research Article

    Induction of Apoptosis by Ethanol Extract of Lythrum anceps (Koehne) Makino in Human Leukemia U937 Cells

    인체백혈병 U937 세포에서 부처꽃 에탄올추출물에 의한 apoptosis 유도

    Jin-Woo Jeong, Chul Hwan Kim, Young-Kyung Lee, Yong Hwang, Ki Won Lee, Kyung-Min Choi, Jung Il Kim

    정진우, 김철환, 이영경, 황용, 이기원, 최경민, 김정일

    Purple loosestrife-Lythrum anceps (Koehne) Makino is a herbaceous perennial plant belonging to the Lythraceae family. It has been used for centuries in ... + READ MORE
    Purple loosestrife-Lythrum anceps (Koehne) Makino is a herbaceous perennial plant belonging to the Lythraceae family. It has been used for centuries in Korea and other Asian traditional medicine. It has been showed pharmacological effects, including anti-oxidant and anti-microbial effects. However, the mechanisms underlying its anti-cancer effect are not yet understood. In this study, we investigated the mechanism of apoptosis signaling pathways by ethanol extract of Lythrum anceps (Koehne) Makino (ELM) in human leukemia U937 cells. Treatment with ELM significantly inhibited cell growth in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies (ApoBDs), DNA fragmentation and increased populations of sub-G1 ratio. Induction of apoptosis by ELM was connected with up-regulation of death receptor (DR) 4 and DR5, pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic Bcl-2 protein, and inhibitor of apoptosis protein (IAP) family proteins, depending on dosage. This induction was associated with Bid truncation, mitochondrial dysfunction, proteolytic activation of caspases (-3, -8 and –9) and cleavage of poly(ADP-ribose) polymerase protein. Therefore, our data indicate that ELM suppresses U937 cell growth by activating the intrinsic and extrinsic apoptosis pathways, and thus may have applications as a potential source for an anti-leukemic chemotherapeutic agent. - COLLAPSE
    August 2020
  • Research Article

    Effects of Ethanol Extract from Lathyrus palustris on Anti-inflammation Response of RAW 264.7 Cell

    RAW 264.7 대식세포 염증반응에 대한 털연리초 에탄올 추출물의 항염증 효과

    Jung Hwan Nam

    남정환

    Lathyrus palustris often used as a treatment for inflammation of the kidneys in Korean traditional medication. Generally, drugs for arthritis have anti-inflammatory ... + READ MORE
    Lathyrus palustris often used as a treatment for inflammation of the kidneys in Korean traditional medication. Generally, drugs for arthritis have anti-inflammatory and antinociceptive properties. However, the validity of the anti-inflammatory effect has not been scientifically investigated so far. Therefore, the purpose of the research was to investigate the latent anti-inflammatory ability of L. palustris using the ethanol extract. To evaluate the anti-inflammatory activities, we examined the inflammatory arbitrators such as a nitric oxide (NO) and prostaglandin E2 (PGE2) on RAW 264.7 cells. Our results indicated that ethanol extract significantly inhibited the lipopolysaccharide E (LPS) derived PGE2 production in RAW 264.7 cell. The inhibitory activity of ethanol extract for PGE2 tests with inhibition ratio showed in 40 ㎍/mL. Overall, PGE2 tests had a higher inhibitory effect on inflammation than NO tests. This result anticipated that the ethanol extract from L. palustris is a good candidate for developing the origin of anti-inflammatory agents. - COLLAPSE
    August 2020
  • Research Article

    Morphological Characteristics of ‘Ilbon’ (Ziziphus jujuba) with Higher Seed Productivity and Functional Component

    종자형성능과 기능성 성분이 높은 대추 유전자원 ‘일본’의 형태적 특성

    Ha Kyung Oh, Sewon Oh, Hyeondae Han, Heesoon Park, Kyeong Hee Lee, Hyunman Shin, Daeil Kim

    오하경, 오세원, 한현대, 박희순, 이경희, 신현만, 김대일

    Morphological characteristics of Ziziphus jujuba accessions, including ‘Ilbon’, were investigated to verify the possibility of ‘Ilbon’ as a breeding material. ‘Ilbon’ has ... + READ MORE
    Morphological characteristics of Ziziphus jujuba accessions, including ‘Ilbon’, were investigated to verify the possibility of ‘Ilbon’ as a breeding material. ‘Ilbon’ has smaller leaves and fruits and a lower total soluble solids content compared to the major Z. jujuba accessions. However, total polyphenol content and antioxidant capacity were highest in ‘Ilbon’ among the Z. jujuba accessions. In addition, ‘Ilbon’ could produce intact seeds and seed productivity of ‘Ilbon’ was highest compared with major Z. jujuba accessions. Principal component analysis and clustering analysis using the morphological characteristics of Z. jujuba accessions have been classified them to geographical origin. In particular, ‘Ilbon’ was clearly distinguished from the major Z. jujuba accessions. Therefore, ‘Ilbon’, which has the highest seed productivity and functional component, will be used as a material for cross breeding and will promote genetic study of Z. jujuba. - COLLAPSE
    August 2020
  • Research Article

    Propagation of Cutting Method of a Rare Endemic Wikstroemia ganpi (Sieb. et Zucc.) Maxim. in Korea

    희귀수종 거문도닥나무(Wikstroemia ganpi (Sieb. et Zucc.) Maxim.)의 번식에 관한 연구

    Jung Won Yoon, Myung Hoon Yi, Jung Won Sung

    윤정원, 이명훈, 성정원

    This study was conducted on propagation use for conservation scheme of a threatened plant; Wikstroemia ganpi (Sieb. et Zucc.) Maxim(Geomundo false ... + READ MORE
    This study was conducted on propagation use for conservation scheme of a threatened plant; Wikstroemia ganpi (Sieb. et Zucc.) Maxim(Geomundo false ohelo). The seed propagation was showed higher (95%) in storage and lower values in ground and cutting. Softwood cutting was higher than hardwood cutting and it was possessing higher ratio of rooting that increase concentration of IBA and NAA. It was determined that for Geomundo false ohelo seedling was effective than cutting. In-situ and ex-situ conservation and restoration of substitute habitats of Geomundo false ohelo is therefore necessary due to human trampling in the habitats, damage, natural selection, loss and suppression. - COLLAPSE
    August 2020
  • Research Article

    Vascular Plant Species in the Southern Sejong

    세종시 남부일대의 관속식물상 연구

    Jung Won Sung, Shin Gu Kang

    성정원, 강신구

    The flora of Sejong were summarized as 531 taxa including 104 families, 315 genera, 462 species, 3 subspecies, 59 varieties and 7 ... + READ MORE
    The flora of Sejong were summarized as 531 taxa including 104 families, 315 genera, 462 species, 3 subspecies, 59 varieties and 7 form. The rare plants were 8 taxa including Nepeta cataria L. and Aristolochia ontorta Bunge. The Korea endemic plants were 9 taxa including Populus tomentiglandulosa T. B. Le and Clematis trichotoma Nakai.. The specific plants were 42 taxa including Phytolacca esculenta Van Houtte(Ⅴ), Corydalis decumbens (Thunb.) Pers(Ⅳ), Poncirus trifoliata Raf(Ⅲ), Pseudostellaria coreana (Nakai) Ohwi(Ⅱ), Cyrtomium fortunei J.Sm.(Ⅰ). The naturalized plants were 49 taxa including Sicyos angulatus L, Ambrosia trifida L, Physalis angulata L. Panicum dichotomiflorum Michx., Galinsoga parviflora Cav, and Erigeron philadelphicus L. Especially, Sicyos angulatus L., Phytolacca americana L. and Bidens frondosa L. have been spread throughout the country are required to manage. The naturalized index(NI) and urbanized index(UI) were 8.7% and 15.3%. Useful plants incuding Edible was 199 taxa, Pasture was 193 taxa, Medicinal was 178 taxa, Ornamental was 82 taxa, Timber was 25 taxa, Fiber was 18 taxa, Stain was 9 taxa. Useful plants will be important materials for plant design in recreational and healing forests. - COLLAPSE
    August 2020
  • Research Article

    The Vascular Plants of Mt. Taegi (Gangwon-do), Korea

    태기산(강원도)의 관속식물상

    Eun-Mi Sun, Dong Chan Son, Hye-Won Kim, Young-Ho Ha, Hee Young Gil, Kang-Hyup Lee, Seung-Hwan Oh

    선은미, 손동찬, 김혜원, 하영호, 길희영, 이강협, 오승환

    Mt. Taegi (1,261.4 m) located in Gangwon-do, is a geographically important region for plant biodiversity. But the biodiversity of Mt ... + READ MORE
    Mt. Taegi (1,261.4 m) located in Gangwon-do, is a geographically important region for plant biodiversity. But the biodiversity of Mt. Taegi has been seriously damaged by the invasion of foreign plants, the development of forests and so on. We conducted 21 field surveys between April and August in 2008 and March and October in 2018 to investigate the vascular flora in Mt. Taegi. Total 662 taxa were identified including 96 families, 338 genera, 580 species, 4 subspecies, 66 varieties and 12 formas. Among them, 28 taxa were Korean endemic plants and 8 taxa were rare plants designated by the Korea Forest Services, and 11 taxa were listed on the Red list of endangered plants and 127 taxa were designated as the floristic regional indicator plants according to the Ministry of Environment. The alien plants were 45 taxa, including R. acetosella that is the ecosystem disturbing species. - COLLAPSE
    August 2020
  • Research Article

    The Vascular Plants of Apsan Park in Daegu, Korea

    대구 앞산공원 일대의 관속식물상

    Jeong Gul Jang, Sung Tae Yoo, Byung Do Kim, Hye Yeon Kwon, Myung Hoon Yi

    장정걸, 유성태, 김병도, 권혜연, 이명훈

    To investigate the distribution of vascular plants growing at Apsan park, we surveyed from April in 2018 to October in 2019. The ... + READ MORE
    To investigate the distribution of vascular plants growing at Apsan park, we surveyed from April in 2018 to October in 2019. The flora of Apsan park was classified as a total of 721 taxa comprising of 114 families, 382 genus, 619 species, 5 subspecies, 77 varieties, 12 forma, 5 hybrid, 3 cultivation. Among them, The Rare plants species 23 taxa, endemic plants 15 taxa, floristic regional indicator plants 98 taxa, naturalized plants 57 taxa were recorded. The percentage of urbanization index was 17.76%, the naturalized ratio was 7.91%, respectively. - COLLAPSE
    August 2020
  • Research Article

    A Short Record for the New Distribution and Some Morphological Characters of Plagiothecium platyphyllum Mönk. (Plagiotheciaceae)

    둥근대이끼(산주목이끼과)의 신분포지 및 형태적 특징

    Gyu-Jin Hwang, Seon-Joo Park

    황규진, 박선주

    Plagiothecium platyphyllum Mönk (Plagiotheciaceae), a species unrecorded previously in the moss flora of the Republic of Korea (South Korea), was collected in ... + READ MORE
    Plagiothecium platyphyllum Mönk (Plagiotheciaceae), a species unrecorded previously in the moss flora of the Republic of Korea (South Korea), was collected in the Surak stream of Mt. Daedun in Jeollabuk-do Wanju-gun Sanbuk-ri. This species was known to be distributed around China, Japan, North Korea, and Europe. The morphological characters of P. platyphyllum’s leaf are ovate-lanceolate, and mostly asymmetric, 2.0~2.5 × 1.0~1.4 ㎜. The margin of the leaf is entirely below and weakly serrulated near the apex. The costae parts are double, unequal, with one branch reaching to the mid-leaf. Median leaf cells are linear-rhomboidal, 80~127 × 10~16 ㎛ and alar cells are rectangular. Central strand is developed in the core of the stem. - COLLAPSE
    August 2020
  • Data

    A Study on the Collection and Utilization of Myanmar Plants as Garden Plants

    정원소재로서의 미얀마 유용 및 관상식물 수집・활용에 관한 연구

    Tai Hyeon Ahn1, Yeo Bin Goh2, Hye Young Jin, Jun Gyu Bae, Ki Cheol Lee

    안태현, 고여빈, 진혜영, 배준규, 이기철

    Studies on the preservation and propagation of tropical and subtropical plants are required, but the studies are insufficient in Korea. Since 2013 ... + READ MORE
    Studies on the preservation and propagation of tropical and subtropical plants are required, but the studies are insufficient in Korea. Since 2013, the Tropical Plant Resources Research Center has been collaborating on field research and publication to preserve plant diversity in Myanmar protected areas and Sagging areas in cooperation with Ministry of Natural Resources and Environment Conservation and the Myanmar Forest Research Institute. This study is to provide information on cooperative projects. As a result of the joint research between the Korea National Arboretum and Myanmar, in December 2018, the Korea National Arboretum Tropical Plant Resources Research Center is on display for conservation and education purposes with the 65 Myanmar useful materials and specimens of 326 kinds of plants and specimens that Myanmar Forest Research Institute donated. This donation is the first case of Myanmar’s export of plant organisms to a foreign country, and as for the Korea National Arboretum, it is the second official case of introducing a mass plant from foreign governments. The study will also accelerate preservation biodiversity and propagation methods of tropical and subtropical plants as well as research on biodiversity conservation and industrial use. - COLLAPSE
    August 2020