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2020 Vol.33, Issue 5 Preview Page

Research Article

Induction of Apoptosis by Sageretia thea Branch Extracts through Activation of NF-κB Signaling Pathway in Human Colorectal Cancer Cells

상동나무(Sageretia thea) 가지추출물의 대장암세포에서 NF-κB 신호전달 활성화를 통한 세포사멸 유도활성

Jeong Dong Kim1
,
Su Bin Park1
,
Hyun Ji Eo2
,
Gwang Hun Park3
,
Jin Boo Jeong4*
김 정동1
,
박 수빈1
,
어 현지2
,
박 광훈3
,
정 진부4*
1Graduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Post-doc, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
3Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
4Professor, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
1국립안동대학교 생약자원학과, 대학원생
2국립산림과학원 산림약용자원연구소, 박사후연구원
3국립산림과학원 산림약용자원연구소, 연구사
4국립안동대학교 생약자원학과, 교수
* Corresponding Author
1 October 2020. pp. 428-435
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Abstract

In this study, we evaluated the inhibitory effect against cell growth and potential molecular mechanism of 100% ethanol extracts of branch from Sageretia thea in human colorectal cancer cells, HCT116. Ethanol dose-dependently extracts of STB significantly suppressed the growth of HCT116 cells through apoptosis. STB activated NF-κB signaling pathway through IκB-α proteasomal degradation and inducing p65 accumulation in nucleus. The inhibition of GSK3β by LiCl didn’t affect STB mediated degradation IκB-α but STB mediated p65 accumulation in nucleus. In addition, STB phosphorylated GSK3β. Based on these findings, STB may be a potential candidate for the development of anti-cancer agents for human colorectal cancer.

Keywords
Apoptosis
Cancer chemoprevention
Colorectal cancer
NF-κB signaling
Sageretia thea
References
1
Arghi, M., I. Soerjomataram, M. Jenkins, J. Brierley, E. Morris, F. Bray and M. Arnold. 2019. Global trends in colorectal cancer mortality: Projections to the year 2035. Int. J. Cancer. 144:2992-3000.
10.1002/ijc.3205530536395
2
Bi, X., Q. Lin, T.W. Foo, S. Joshi, T. You, H.M. Shen, C.N. Ong, P.Y. Cheah, K.W. Eu and C.L. Hew. 2006. Proteomic analysis of colorectal cancer reveals alterations in metabolic pathways. Mol. Cell. Proteom. 5(6):1119-1130.
10.1074/mcp.M500432-MCP20016554294
3
Bours, V., M.B. Alj, A.C. Hellin, P. Viatour, P. Robe, S. Delhalle, V. Benoit and M.P. Merville. 2000. Nuclear factor-κB, Cancer, and Apoptosis. Biochem. Pharmacol. 60:1085-1090.
10.1016/S0006-2952(00)00391-9
4
Bray, F., J. Ferlay, I. Soerjomataram, R.L. Siegel, L.A. Torre and A. Jemal. 2018. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 68:394-424.
10.3322/caac.2149230207593
5
Cheng, Y.Y., C.H. Hsieh, T.H. Tsai. 2018. Concurrent administration of anticancer chemotherapy drug and herbal medicine on the perspective of pharmacokinetics. J. Food Drug Anal. 26:S88-S95.
10.1016/j.jfda.2018.01.00329703390
6
Dolcet, X., D. Llobet, J. Pallares and X. Matias-Guiu. 2005. NF-κB in development and progression of human cancer. Virchows Arch. 446:475-482.
10.1007/s00428-005-1264-915856292
7
Hassanzadeh, P. 2011. Colorectal cancer and NF-κB signaling pathway. Gastroenterol. Hepatol. Bed Bench 4(3):127-132.
8
Hoeflich, K.P., J. Luo, E.A. Rubie, M.S. Tsao, O. Jin and J.R. Woodgett. 2000. Requirement for glycogen synthase kinase-3β in cell survival and NF-κB activation. Nature 406:86-90.
10.1038/3501757410894547
9
Hyun, T.K., S.C. Song, C.K. Song and J.S. Kim. 2015. Nutritional and nutraceutical characteristics of Sageretia theezans fruit. J. Food Drug Anal. 23:742-749.
10.1016/j.jfda.2015.04.00628911491
10
Irmak, Z., O. Tanriverdi, H. Odemis and D.D. Uysal. 2019. Use of complementary and alternative medicine and quality of life of cancer patients who received chemotherapy in Turkey. Complement. Ther. Med. 44:143-150.
10.1016/j.ctim.2019.04.00831126547
11
Jeong, J.B., X. Yang, R. Clark, J.E. Choi, S.J Baek and S.H. Lee. 2013. A mechanistic study of the proapoptotic effect of tolfenamic acid: involvement of NF-κB activation. Carcinogenesis 34(10):2350-2360.
10.1093/carcin/bgt22423784084
12
Jung, K.W., Y.J. Won, H.J. Kong and E.S. Lee. 2018. Cancer statistics in Korea: Incidence, mortality, survival, and prevalence in 2015. Cancer Res. Treat. 50(2):303-316.
10.4143/crt.2018.14329566481PMC5912151
13
Kim, H.N., G.H. Park, J.D. Kim, S.B. Park, H.J. Eo and J.B. Jeong. 2019a. Effect of the extracts from the leaves and branches of Sageretia thea on β-catenin proteasomal degradation in human colorectal and lung cancer cells. Korean J. Plant Res. 32(2):153-159.
14
Kim, H.N., G.H. Park, S.B. Park, J.D. Kim, H.J. Eo, H.J. Son, J.H. Song and J.B. Jeong. 2019b. Extracts from Sageretia thea reduce cell viability through inducing cyclin D1 proteasomal degradation and HO-1 expression in human colorectal cancer cells. BMC Complement. Altern. Med. 19:43.
10.1186/s12906-019-2453-430736789PMC6368743
15
Kim, H.N., G.H. Park, S.B. Park, J.D. Kim, H.J. Eo, H.J. Son, J.H. Song and J.B. Jeong. 2019c. Sageretia thea inhibits inflammation through suppression of NF-κB and MAPK and activation of Nrf2/HO-1 signaling pathways in RAW264.7 cells. Am. J. Chin. Med. 47(2)385-403.
10.1142/S0192415X1950019830834779
16
Kole, L., B. Giri, S.K. Manna, B. Pal and S. Ghosh. 2011. Biochanin-A, an isoflavone, showed anti-proliferative and anti-inflammatory activities through the inhibition of iNOS expression, p38-MAPK and ATF-2 phosphorylation and blocking NFκB nuclear translocation. Eur. J. Pharmacol. 653:8-15.
10.1016/j.ejphar.2010.11.02621147093
17
Lin, Y., L. Bai, W. Chen, and S. Xu. 2010. The NF-κB activation pathways, emerging molecular targets for cancer prevention and therapy. Expert Opin. Ther. Targets 14(1):45-55.
10.1517/1472822090343106920001209PMC3043547
18
Meyerhardt, J.A. and R.J. Mayer. 2005. Systemic therapy for colorectal cancer. N. Engl. J. Med. 352:476-87.
10.1056/NEJMra04095815689586
19
Moon, S.M., S.A. Lee, J.H. Hong, J.S. Kim, D.K. Kim and C.S. Kim. 2018. Oleamide suppresses inflammatory responses in LPS-induced RAW264.7 murine macrophages and alleviates paw edema in a carrageenan-induced inflammatory rat model. Int. Immunopharmacol. 56:179-185.
10.1016/j.intimp.2018.01.03229414648
20
Park, S.B., G.H. Park, Y.R. Um, H.N. Kim, H.M. Song, N.H. Kim, H.S. Kim and J.B. Jeong. 2018. Wood-cultivated ginseng exerts anti-inflammatory effect in LPS-stimulated RAW264.7 cells. Int. J. Biol. Macromol. 116:327-334.
10.1016/j.ijbiomac.2018.05.03929751039
21
Perkins, N.D. and T.D. Gilmore. 2006. Good cop, bad cop: The different faces of NF-κB. Cell Death Differ. 13:759-772.
10.1038/sj.cdd.440183816410803
22
Pfeffer, C.M. and A.T.K. Singh. 2018. Apoptosis: A target for anticancer therapy. Int. J. Mol. Sci. 19(2):448.
10.3390/ijms1902044829393886PMC5855670
23
Wang, H., T.O. Khor, L. Shu, Z. Su, F. Fuentes, J.H. Lee and A.N.T. Kong. 2012. Plants against cancer: A review on natural phytochemicals in preventing and treating cancers and their druggability. Anti-Cancer Agents Med. Chem. 12:1281-1305.
10.2174/18715201280383302622583408
24
Wyllie, A.H., G.J. Beattie and A.D. Hargreaves. 1981. Chromatin changes in apoptosis. Histochem J. 681-692.
10.1007/BF010027196975767
25
Yaghoubi, N., A. Soltani, K. Ghazvini, S.M. Hassanian and S.I. Hashemy. 2019. PD-1/ PD-L1 blockade as a novel treatment for colorectal cancer. Biomed. Pharmacother. 110:312-318.
10.1016/j.biopha.2018.11.10530522017
26
Yun, S.I., H.Y. Yoon, S.Y. Jeong and Y.S. Chung. 2009. Glucocorticoid induces apoptosis of osteoblast cells through the activation of glycogen synthase kinase 3β. J. Bone Miner. Metab. 27:140-148.
10.1007/s00774-008-0019-519066717
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :5
  • Pages :428-435
  • Received Date : 2020-07-03
  • Revised Date : 2020-07-25
  • Accepted Date : 2020-07-28
  • DOI :https://doi.org/10.7732/kjpr.2020.33.5.428
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