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2019 Vol.32, Issue 4

Research Article

Induction of Apoptosis by Water Extract of Glycyrrhizae radix in Human Bladder T24 Cancer Cells

인체 방광암 T24 세포에서 감초(Glycyrrhizae radix) 열수추출물에 의한 apoptosis 유도

Ki Won Lee1
,
Jeong Il Kim2
,
Seung Young Lee3
,
Kyung-Min Choi3
,
Young Taek Oh4
,
Jin-Woo Jeong4*
이 기원1
,
김 정일2
,
이 승영3
,
최 경민3
,
오 영택4
,
정 진우4*
1Researcher, Kwangdong Pharm Co., Ltd, Seoul 08381, Korea
2Professor, Department of Occupational and Environmental Medicine, Busan 49201, Korea
3Researcher, Performance Management Division, Nakdonggang National Institute of Biological Resources, Sangju-si 37242, Korea
4Researcher, Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources, Sangju-si 37242, Korea
1광동제약(주), 연구원
2동아대학교 의과대학 직업환경의학교실, 교수
3국립낙동강생물자원관 혁신성과부, 연구원
4국립낙동강생물자원관 동식물활용연구팀, 연구원
* Corresponding Author
31 August 2019. pp. 255-263
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Abstract

Glycyrrhizae radix is one of the most frequently prescribed ingredients in Oriental medicine, and Glycyrrhizae radix extract has been shown to exert anti-cancer effects. However, the cellular and molecular mechanisms of programed cell death (apoptosis) by Glycyrrhizae radix are poorly defined. In the present study, it was examined the molecular mechanisms of apoptosis by water extracts of Glycyrrhizae radix (GRW) in human bladder T24 cancer cells. It was found that GRW could inhibit the cell growth of T24 cells in a concentration-dependent manner, which was associated with the induction of apoptotic cell death, as evidenced by the formation of apoptotic bodies, DNA fragmentation and increased populations of annexin-V positive cells. The induction of apoptotic cell death by GRW was connected with an up-regulation of pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic proteins (Bcl-2 and Bcl-xL), and inhibition of apoptosis family proteins (XIAP, cIAP-1 and cIAP-2). In addition, apoptosis-inducing concentrations of GRW induced the activation of caspase-9, an initiator caspase of the mitochondrial-mediated intrinsic pathway, and caspase-3, accompanied by proteolytic degradation of PARP. GRW also induced apoptosis via a death receptor-mediated extrinsic pathway by caspase-8 activation, resulting in the down-regulation of total Bid and suggesting the existence of cross-talk between the extrinsic and intrinsic pathways. Taken together, the present results suggest that GRW may be a potential chemotherapeutic agent for the control of human bladder cancer cells.

Keywords
Apoptosis
Caspase
Glycyrrhizae radix
T24 cells
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :4
  • Pages :255-263
  • Received Date : 2019-03-08
  • Revised Date : 2019-04-11
  • Accepted Date : 2019-05-14
  • DOI :https://doi.org/10.7732/kjpr.2019.32.4.255
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