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2019 Vol.32, Issue 5 Preview Page

Research Article

Analysis of Antioxidant Activity and Serotonin Derivatives in Safflower (Carthamus tinctorius L.) Germplasm Collected from Five Countries

국외 수집 홍화 유전자원의 항산화 활성 및 세로토닌 유도체 함량 분석

Yi Jin Jung1
,
Awraris Derbie Assefa2
,
Jae Eun Lee3
,
Ho Sun Lee4
,
Ju Hee Rhee4
,
Jung Sook Sung5*
정 이진1
,
아와리스 아세파2
,
이 재은3
,
이 호선4
,
이 주희4
,
성 정숙5*
1Research Assistant, National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea
2Post-doc, National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea
3Researcher, National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea
4Senior Researcher, National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea
5Senior Researcher, Upland Crop Breeding, Research Division Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
1농촌진흥청 국립농업과학원 농업유전자원센터, 연구원
2농촌진흥청 국립농업과학원 농업유전자원센터, 박사후연구원
3농촌진흥청 국립농업과학원 농업유전자원센터, 농업연구사
4농촌진흥청 국립농업과학원 농업유전자원센터, 농업연구관
5농촌진흥청 국립식량과학원 남부작물부 밭작물개발과, 농업연구관
* Corresponding Author
31 October 2019. pp. 423-432
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Abstract

In order to select potential plant resources as functional materials and natural antioxidants, we evaluated antioxidant activity and serotonin derivatives of safflower germplasm collected from five countries. N-(p-Coumaroyl) serotonin (CS) and N-feruloylserotonin (FS) were analyzed by using Ultra Performance Liquid Chromatography (UPLC). Total polyphenol content (TPC) was determined by Folin-Ciocalteu method and antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), Ferric reducing antioxidant power (FRAP) and Reducing power (RP) assays. The TPC showed a range of 28.25 to 90.53㎍ gallic acid equivalent (GAE)/㎎ dried extract (DE). DPPH, ABTS, FRAP and RP assay were in the range of 18.76 to 93.98, 48.91 to 163.73, 3.80 to 132.29 and 26.32 to 80.08 ㎍ ascorbic acid equivalent (ASCE)/㎎ DE, respectively. Among the five countries, safflower seed collected from Iran had the highest levels of serotonin derivatives and antioxidant activities than other countries (p<0.05). CS showed high correlation with TPC, ABTS and DPPH (r=0.673,0.727,0.820), and FS showed high correlation with DPPH (r=0.740). Accessions IT321214 and IT321215 could be useful for development of new functional materials and could be used as a source of valuable natural antioxidant materials.

Keywords
Antioxidants
Correlation
N-feruloylserotonin
N-(p-Coumaroyl) serotonin
Saffower seed
References
1
Arnao, M.B. 2000. Some methodological problems in the determination of antioxidant activity using chromogen radicals: A Practical Case. J. Trends Food Sci. Technol. 11:419-421.
10.1016/S0924-2244(01)00027-9
2
Bae, S.J., S.M. Shim, Y.J. Park, J.Y. Lee, E.J. Chang and S.W. Choi. 2002. Cytotoxicity of phenolic compounds isolated from seeds of safflower (Carthamus tinctorius L.) on cancer cell lines. J. Food Sci. Biotechnol. 11:140-146 (in Korean).
3
Baek, N.I., M.H. Bang, J.C. Song, S.Y. Lee and N.K. Park. 1999. N-feruloylserotonin, antioxidative component from the seed of Carthamus tinctorius L. J. Korean Soc. Agric. Chem. Biotechnol. 42:366-368 (in Korean).
4
Benzie, I.F. and J.J. Strain. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant power": The Frap Assay. J. Anal. Biochem. 239:70-76.
10.1006/abio.1996.02928660627
5
Branen, A.L. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. American Oil Chem. Soc. 52:59-63.
10.1007/BF02901825805808
6
Cheeseman, K.H. and T.F. Slater. 1993. An introduction to free radical biochemistry. Br Med Bull. 49(3):48-493.
10.1093/oxfordjournals.bmb.a0726258221017
7
Cho, S.H., Y.Y. Park, J.Y. Yoon, S.W. Choi and T.Y. Ha. 2006. The effect of polyphenols from safflower seed on HMG- CoA reductase (HMGR) activity, LDL oxidation and apo A1 secretion. Kor. J. Food Sci. Technol. 38:279-283 (in Korean).
8
Choi, C.H., H.D. Kim, and E.B. Im. 2011. Reviews of research trends on safflower seed (Carthamus tinctorius L.). J. KMC. 24:63-90 (in Korean).
9
Hotta, Y., A. Nagatsu, W. Liu, T. Muto, C. Narumiya, X. Lu, M. Yajima, N. Ishikawa, K. Miyazeki and N. Kawai. 2002. Protective effects of antioxidative serotonin derivatives isolated from safflower against postischemic myocardial dysfunction. J. Mol. Cell. Biochem. 238:151-162.
10.1023/A:101999212498612349903
10
Ito, N., S. Fukushima, A. Haqlwara, M. Shibata and T. Ogiso. 1983. Carcinogenicity of butylated hydroxyanisole in F344 rats. J. Natl. Cancer Inst. 70:343-352.
11
Jang, T.W., S.H. Nam and J.H. Park. 2016. Antioxidant activity and inhibitory effect on oxidative DNA damage of ethyl acetate fractions extracted from cone of red pine (Pinus densiflora). Korean J. Plant Res. 29:163-170 (in Korean).
10.7732/kjpr.2016.29.2.163
12
Kim, D.H., E.Y. Hwang and J.H. Son. 2013. Antiinflammatory activity of carthamus tinctorious seed extracts in raw 264.7 cells. J. Life Sci. 23:55-62 (in Korean).
10.5352/JLS.2013.23.1.55
13
Kim, E.O., K.T. Lee and S.W. Choi. 2008. Chemical comparison of germinated and ungerminated safflower (Carthamus tinctorius) seeds. J. Kor. Soc. Food Sci. Nutr. 37:1162-1167 (in Korean).
10.3746/jkfn.2008.37.9.1162
14
Kim, J.H., J.K. Kim, W.W. Kang, Y.S. Ha, S.W. Choi and K.D. Moon. 2003. Chemical compositions and dpph radical scavenger activity in different sections of safflower. J. Kor. Soc. Food Sci. Nutr. 32:733-738 (in Korean).
10.3746/jkfn.2003.32.5.733
15
Kim, Y.O., S.W. Lee, S.O. Yang, S.W. Na, S.K. Kim and J.H. Chung. 2014. Neuroprotective effects of the extracts from the aerial parts of Carthamus tinctorius L. on transient cerebral global ischemia in rats. Kor. J. Med. Crop Sci. 22(1):46−52 (in Korean).
10.7783/KJMCS.2014.22.1.46
16
Koyama, N., K. Kuribayashi, T. Seki, K. Kobayashi, Y. Furuhata, K. Suzuki, H. Arisaka, T. Nakano, Y. Amino and K. Ishii. 2006. Serotonin derivatives, major safflower (Carthamus tinctorius L.) seed antioxidants, inhibit low density lipoprotein (LDL) oxidation and atherosclerosis in apolipoprotein E-deficient mice. J. Aric. Food Chem. 54:4970-4976.
10.1021/jf060254p16819904
17
Kumar, A., P. Pant, S. Basu, G. Rao and H. Khanna. 2006. Oxidative stress in neonatal hyperbilirubinemia. J. Trop. Pediatr. 53:69-71.
10.1093/tropej/fml06017158814
18
Lee, M.K., J.S. Park, H.J. Song and S.U. Chon. 2014a. Effects of polyphenol and catechin levels on antioxidant activity of several edible flower extracts. Korean J. Plant Res. 27(2): 111-118 (in Korean).
10.7732/kjpr.2014.27.2.111
19
Lee, Y.J., J.H. Kim, J.W. Oh, G.H. Shin, J.S. Lee, J.H. Cho, J.J. Park, J.H. Lim and O.H. Lee. 2014b. Antioxidant and anti-adipogenic effects of kohlrabi and radish sprout extracts. Kor. J. Food Sci. Technol. 46:531-537 (in Korean).
10.9721/KJFST.2014.46.5.531
20
Lobo, V., A. Patil, A. Phatak and N. Chandra. 2010. Free radicals, antioxidants and functional foods: Impact on Human Health. J Pharmacogn. Rev. 4:118.
10.4103/0973-7847.7090222228951PMC3249911
21
Mujic, I., E. Sertovic, S. Jokic, Z. Saric, V. Alibabic, S. Vidovic and J. Zivkovic. 2011. Isoflavone content and antioxidant properties of soybean seeds. Croatian J. Food Sci. Technol. 3:16-20.
22
Nho, J.H., M.O. Sim, H.K. Jung, M.J. Lee, J.H. Jang, D.E. Jung, T.K. Sung, B.K. An and H.W. Cho. 2018. Anti-colorectal cancer and anti-oxidant activities of Rubiae radix ethanol extract in vitro. Korean J. Plant Res. 31(2):102-108 (in Korean).
23
Ou, B., D. Huang, M. Hampsch-Woodill, J.A. Flanagan and E.K. Deemer. 2002. Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: A Comparative Study. J. Aric. Food Chem. 50:3122-3128.
10.1021/jf011660612009973
24
Peng, X.R., X. Wang, J.R. Dong, X.J. Qin, Z.R. Li, H. Yang, L. Zhou and M.H. Qiu. 2017. Rare hybrid dimers with anti- acetylcholinesterase activities from a safflower (Carthamus tinctorius L.) seed oil cake. J. Aric. Food Chem. 65(43): 9453-9459.
10.1021/acs.jafc.7b0343128992692
25
Perron, N.R. and J.L. Brumaghim. 2009. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell. Biochem. Biophys. 53:75-100.
10.1007/s12013-009-9043-x19184542
26
Piao, X.M., E.K. Jang, J.W. Chung, G.A. Lee, H.S. Lee, J.S. Sung, Y.A. Jeon, J.R. Lee, Y.G. Kim and S.Y. Lee. 2013. Variation in antioxidant activity and polyphenol content in tomato stems and leaves. Plant Breed.Biotech.1:366-373 (in Korean).
10.9787/PBB.2013.1.4.366
27
Qazi, N., R.A. Khan and G.H. Rizwani. 2015. Evaluation of antianxiety and antidepressant properties of Carthamus tinctorius L. (safflower) petal extract. Pak J Pharm Sci. 28.3: 991-5.
28
Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol. Med. 26:1231-1237.
10.1016/S0891-5849(98)00315-3
29
Sakamura, S., Y. Terayama, S. Kawakatsu, A. Ichihara and H. Saito. 1978. Conjugated serotonins related to cathartic activity in safflower seeds (Carthamus tinctorius L.). Agric Biol. Chem. 42:1805-1806.
10.1271/bbb1961.42.1805
30
Song, J.C., N.K. Park, H.S. Hur, M.H. Bang and N.I. Baek. 2000. Examination and isolation of natural antioxidants from Korean medicinal plants. Kor. J. Med. Crop Sci. 8:94-101 (in Korean).
31
Sun, T. and S. Tanumihardjo. 2007. An integrated approach to evaluate food antioxidant capacity. J. Food Sci. 72:159-165.
10.1111/j.1750-3841.2007.00552.x18034745
32
Sung, J.S., Y.J. Jeong, D.J. Kim, A.D. Assefa, Y.A. Jeon, O.S. Hur, N.Y. Ro, H.C. Ko, H.C. Ok, J.H. Rhee, M.C. Lee and H.J. Baek. 2018. Oil compositions and antioxidant properties of safflower germplasm collected from east asia. Kor. J. Med. Crop Sci. 26:32-41 (in Korean).
33
Takahashi, T. and M. Miyazawa. 2012. Potent α-glucosidase inhibitors from safflower (Carthamus tinctorius L.) seed. Phytotherapy Research 26(5):722-726.
10.1002/ptr.362222021176
34
Waterhouse, A.L. 2002. Determination of total phenolics: Current Protocols in Food Analytical Chemistry. John Wiley & Sons, Inc., New York, USA.
10.1002/0471142913.fai0101s06
35
Wei, X., H. Liu, X. Sun, F. Fu, X. Zhang, J. Wang, J. An and H. Ding. 2005. Hydroxysafflor yellow A protects rat brains against ischemia-reperfusion injury by antioxidant action. Neurosci. Lett. 386:58-62.
10.1016/j.neulet.2005.05.06916002219
36
Yen, G.C. and H.Y. Chen. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Aric. Food Chem. 43:27-32.
10.1021/jf00049a007
37
Yoo, K.M., D.O. Kim and C.Y. Lee. 2007. Evaluation of different methods of antioxidant measurement. Food Sci. Biotechnol. 16:7-11 (in Korean).
38
Yu, J.M. and H.I. Moon. 2018. Antioxidants and acetyl- cholinesterase inhibitory activity of solvent fractions extracts from Dendropanax morbiferus. Korean J. Plant Res. 31(1): 10-15 (in Korean).
39
Yu, S.Y., Y.J. Lee, J.D. Kim, S.N. Kang, S.K. Lee, J.Y. Jang, H.K. Lee, J.H. Lim and O.H. Lee. 2013. Phenolic composition, antioxidant activity and anti-adipogenic effect of hot water extract from safflower (Carthamus tinctorius L.) seed. Nutrients 5(12):4894-4907.
10.3390/nu512489424288028PMC3875912
40
Zhang, H.L. A. Nagatsu, T. Watanabe, J. Sakakibara and H. Okuyama. 1997. Antioxidative compounds isolated from safflower (Carthamus tinctorius L.) oil cake. Chem. Pharm. Bull. 45:1910-1914.
10.1248/cpb.45.19109433760
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :5
  • Pages :423-432
  • Received Date : 2019-05-23
  • Revised Date : 2019-08-08
  • Accepted Date : 2019-10-17
  • DOI :https://doi.org/10.7732/kjpr.2019.32.5.423
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