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2018 Vol.31, Issue 6 Preview Page

Research Article

Whitening Activity of Abeliophyllum distichum Nakai Leaves According to the Ratio of Prethanol A in the Extracts
Tae-Won Jang1
,
Ji-Soo Choi2
,
Hoi-Ki Kim3
,
Eun-Ja Lee3
,
Man-Wook Han3
,
Ki-Beom Lee4
,
Do-Wan Kim5
,
Jae-Ho Park6*
1Graduate Student, Department of Medicinal Plant Resources, Andong National University
2Graduate Student, Department of Medicinal Plant Science, Jungwon University
3Researcher, Fanipinkorea Co., Ltd
4Senior Researcher, Incheon Business Information Technopark. Biotechnology & Business Center
5Professor, Department of Food Science and Industry, Jungwon University
6Professor, Department of Pharmaceutical Science, Jungwon University
*Corresponding Author
31 December 2018. pp. 667-674
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Abstract

In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichum Nakai. The extracts were prepared using 0, 50, 70, and 100% prethanol A at 121℃, 1.2 atm for 15 minutes. To confirm effective extraction, the acteoside content of each extract was analyzed with the HPLC-PDA method. The antioxidant activity was evaluated using DPPH and ABTS scavenging activity assays, and the whitening activity was evaluated based on inhibitory activities on the protein and mRNA expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16 F10 cells. Each extract showed strong antioxidant and whitening activity. IC50 values of antioxidant activity from each extract were in order of 100%, 70%, 50%, and 0%. In addition, whitening activity inhibited the protein and mRNA expression of melanin synthesis factor, following the same pattern as antioxidant activity. In conclusion, water and prethanol A extracts of A. distichum showed effective antioxidant and whitening activity and are thus considered to be valuable materials for whitening cosmetics. The results of this study will also provide basic data for the safe and efficient production of A. distichum as a cosmetic material.

Keywords
Abeliophyllum distichum
MITF
TRP-1
TRP-2
Tyrosinase
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :667-674
  • Received Date : 2018-10-30
  • Revised Date : 2018-12-06
  • Accepted Date : 2018-12-12
  • DOI :https://doi.org/10.7732/kjpr.2018.31.6.667
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