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2018 Vol.31, Issue 5 Preview Page

Research Article

Induction of A Chromosome-doubled Persimmon (Diospyros kaki Thunb.) by in vitro Colchicine Treatment

기내 콜히친 처리에 의한 염색체 배가 감 식물체 유기

Kyeong-Bok Ma1*
,
Kwang-Sik Cho2
,
Hae-Won Jung1
,
Ho-Jin Seo3
,
Sam-Seok Kang34
마 경복1*
,
조 광식2
,
정 해원1
,
서 호진3
,
강 삼석34
1Researcher, Pear Research Station, National Institute of Horticultural & Herbal Science
2Researcher, Pear Research Station, National Institute of Horticultural & Herbal Science
3Post-doc, Pear Research Station, National Institute of Horticultural & Herbal Science
4Senior Researcher, Pear Research Station, National Institute of Horticultural & Herbal Science
1국립원예특작과학원 배연구소, 농업연구사
2국립원예특작과학원 배연구소, 연구원
3국립원예특작과학원 배연구소, 박사연구원
4국립원예특작과학원 배연구소, 농업연구관
* Corresponding Author
31 October 2018. pp. 515-521
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Abstract

This was carried out to develop a chromosome-doubled (12x) persimmon that will be used as a crossing parent to select seedless persimmon cultivars with the change of the consumption trend recently. To obtain a chromosome-doubled (12x) persimmon, colchicine was applied at the meristem of seedlings in vitro derived from cross among hexaploid persimmon (Diopyros kaki Thunb.). These were treated with 0.03%, 0.05% and 0.1% colchicine respectively for doubling chromosome, and it was most effective at the concentration of 0.05% colchicine. After colchicine treatment, we conducted tests to elucidate conditions for inducing shoot and root development. As the result, the shoots grew best when cultivated at 1/2MS media plus 10 and 30 μM zeatin respectively, and the roots grew best when cultivated at 1/2MS media after dipping for 5 seconds at 10 mM NAA+5% DMSO. We also compared seedlings that have chromosome (6x) do not doubled and crossing parents (6x) and chromosome-doubled seedlings (12x). As the result, these chromosome-doubled seedlings (12x) showed lower stomatal density and larger stomatal size.

Keywords
Chromosome doubling
Colchicine treatment
Embryo culture
Persimmon
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :5
  • Pages :515-521
  • Received Date : 2018-06-05
  • Revised Date : 2018-07-05
  • Accepted Date : 2018-07-24
  • DOI :https://doi.org/10.7732/kjpr.2018.31.5.515
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