All Issue

2018 Vol.31, Issue 2

Research Article

Role of Iridin Isolated from Iris koreana Nakai on Doxorubicin-induced Necrosis in HK-2 Cells, and Effect on Cancer Cells

노랑붓꽃에서 분리된 Iridin의 독소루비신 유도 HK-2 세포 괴사에 대한 역할 및 암세포에 대한 작용

Jong Hyun Nho1*
,
Ki Ho Lee1*
,
Ho Kyung Jung1
,
Mu Jin Lee1
,
Ji Hun Jang1
,
Mi Ok Sim1
,
Ja Kyun Jung1
,
Da Eun Jung1
,
Hyun Woo Cho1**
노 종현1*
,
이 기호1*
,
정 호경1
,
이 무진1
,
장 지훈1
,
심 미옥1
,
정 자균1
,
정 다은1
,
조 현우1**
1National Development Institute of Korean Medicine, Jangheung-gun 59338, Korea
1한약진흥재단
* First Author
** Corresponding Author
30 April 2018. pp. 95-101
PDF XML Citation
Abstract

Doxorubicin is a anti-cancer drugs that interferes with the growth and spread of cancer cells in human body. Doxorubicin is used to treat different types of cancers that affect the ovary, thyoid and lungs, but induced side effect such as nephrotoxicity and cardiotoxicity. Thus, we investigated that the effect of iridin on doxorubicin-induced necrosis in HK-2 cells, a human proximal tubule cell. To confirm effect of iridin on doxorubicin-induced necrosis, HK-2 cells are treated with 10 μM doxorubicin and 80 μM iridin. 80 μM iridin reduced 10 μM doxorubicin-induced necrosis, the mitochondrial over activation and caspase-3 activation. However, iridin reduces anti-cancer effect of doxorubicin such as PARP1 and caspase-3 activation, checkpoint proteins (CDK4 and CDK6) in NCI-H1129 cells (Human non-small cell lung cancer cell). In HCT-116 cells (Human colorectan cancer cell), iridin do not increased protein expression of CDK4 and CDK6 decreased by doxorubicin. Results indicate that treatment of iridin was diminished doxorubicin-induced necrosis in HK-2 cells. However, iridin was decreased anti-cancer effect of doxorubicin on NCI-H1229, but not HCT-116. Thus, further experiment are required to iridin treatment on various cancer cells and animal models because effect of iridin different cell type.

Keywords
Doxorubicin
HK-2
Iridin
Iris koreana Nakai
Necrosis
Nephrotoxicity
References
1
Choi, K.M., J.H. Lee, G.O. Adam, S.J. Kim, H.S. Kang, Y.S. Yang and G.B. Kim. 2017. Effects of the Jinan red ginseng extract treatment on poloxamer 407-induced hyperlipidemia in rabbits. Korean J. Plant Res. 30:601-611.
2
Eom, T.H., J.H. Kim, S.I. Lee and J.G. Jeong. 2013. A herbalogical study on the plants of Iridaceae in Korea. Korean J Herbol. 28:85-93.
10.6116/kjh.2013.28.3.85
3
Guo, H.F. and M.H. Wang. 2017. Anti-inflammatory and anti- cancer effect of Stachys affinis tubers. Korean J. Plant Res. 30:679-685.
4
Kim, Y.S. and J.H. Cho. 2016. Optimal treatment of advanced pancreatic neuroendocrine tumor. Korean J Pancreas Biliary Tract. 21:128-137.
10.15279/kpba.2016.21.3.128
5
Lee, J.H., C.H. Lee, G.W. Park and C.Y. Song. 2007. Effect of forcing date and temperature on growth and flowering of Iris koreana and Iris minutoaurea. J Korean For Soc. 96:699- 704.
6
Lee, H.J. and S.J. Park. 2013. A phylogenetic study of Korean Iris L. based on pastid DNA (psbA-trnH, trnL-F) sequences. Korean J PI Taxon. 43:227-235.
10.11110/kjpt.2013.43.3.227
7
Marcos, M. 2014. Cyclin-dependent kinases. Genome Biol. 15:122.
10.1186/gb4184PMC4097832
8
Park, H.J., S.J. Jin, Y.N. Oh, S.G. Yun, J.Y. Lee, H.J. Kwon and B.W. Kim. 2013. Induction of G1 arrest by methanol extract of Lycopus lucidus in human lung adenocarcinoma A549 cells. J Life Sci. 23:1109-1117.
10.5352/JLS.2013.23.9.1109
9
Pi, J.H., J.G. Park, J.Y. Jung, J.S. Park, G.U. Suh and S.W. Son. 2016A. Habitats environmental and population characteristics of Iris koreana Nakia, a rare and endemic species in Korea. J Agric & Life Sci. 49:102-109.
10
Pi, J.H., J.G. Park, J.Y. Jung, J.S., Park, H.H., Yang, G.U., Suh, C.H. Lee and S.W. Son. 2016B. Vegetation structure and flora of Iris koreana Nakia, endemic species in Korea. J Agric & Life Sci. 50:55-67.
10.14397/jals.2016.50.6.55
11
Park, G.H., H.M Song, S.B. Park, J.H. Park, M.S. Shin, H.J. Son, Y. Um and J.B. Jeong. 2017. Anti-proliferative activity of ethanol extracts from Taxilli ramulus (Taxillus chinensis (DC.) Danser) through cyclin D1 proteasomal degradation in human colorectal cancer cells. Korean J. Plant Res. 30:640-646.
12
Shin, H.J., H.K. Kwon, J.H. Lee, X. Gui, A. Achek, J.H. Kim and S. Choi. 2015. Doxorubicin-induced necrosis is mediated by poly (ADP-ribose)polymerase 1 (PARP1) but is independent of p53. Sci Rep. 5:15798.
10.1038/srep1579826522181PMC4629133
13
Thorn, C.F., C. Oshiro, S. Marsh, T. Hernandez-Boussard, H. Mcleod, T.E. Klein and R.B. Altman. 2011. Doxorubicin pathways: pharmacodynamics and adverse effects. Phamacogenet Genomics 21:440-446.
10.1097/FPC.0b013e32833ffb5621048526PMC3116111
14
Tacar, O., P. Sriamornsak and C.R. Dass. 2013. Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems. J Pharm Pharmacol. 65:157-170.
10.1111/j.2042-7158.2012.01567.x23278683
15
Tarasewicz, E., R. Hamdan, J. Straehla, A. Hardy, O. Nunez, S. Zelivianski, D. Dokic and J.S. Jeruss. 2014. CDK4 inhibition and doxorubicin mediate breast cancer cell apoptosis through Smad3 and survivin. Cancer Biol Ther. 15:1301-1311.
10.4161/cbt.2969325006666PMC4130723
16
Tadesse, S., M. Yu, M. Kumarasiri, B.T. Le and S. Wang. 2015. Targeting CDK6 in cancer: State of the art and new insights. Cell Cycle 14:3220-3230.
10.1080/15384101.2015.108444526315616PMC4825601
17
Yoon, G.A., S.Y. Chae and K.H. Kim. 2012. Synergistic anticancer activity on mouse sarcoma by mixture of doxorubicin and water extract of Albizzia julibrissin. Cancer Prev Res. 17:239-243.
18
Yoon, J.H., S.H. Sohn, E.Y. Lee, G.S. Kim, S.E. Lee, D.Y. Lee, K.H. Seo, S.W. Lee and H.D. Kim. 2015. Protective effect of saururus chinensis ethanol extract against styrene in mouse spermatocyte cell line. Korean J Med Crop Sci. 25:45-51.
10.7783/KJMCS.2017.25.1.45
19
Yuan, J., A. Najafov and B.F. Py. 2016. Roles of caspases in necrotic cell death. Cell 15:1693-1704.
10.1016/j.cell.2016.11.04727984721PMC5381727
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :2
  • Pages :95-101
  • Received Date : 2017-08-07
  • Revised Date : 2017-11-17
  • Accepted Date : 2017-12-12
  • DOI :https://doi.org/10.7732/kjpr.2018.31.2.095
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close