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2019 Vol.32, Issue 2

Research Article

Inhibitory Effect of the Branch Extracts from Taxillus yadoriki Parasitic to Neolitsea sericea against the Cell Proliferation in Human Lung Cancer Cells, A549

참식나무(Neolitsea sericea) 기주 참나무겨우살이(Taxillus yadoriki) 가지 추출물의 폐암세포 A549에 대한 세포생육 억제활성

Su Bin Park1
,
Ha Na Kim1
,
Jeong Dong Kim2
,
Gwang Hun Park3
,
Hyun Ji Eo4
,
Mi-Yun An1
,
Jin Boo Jeong5*
박 수빈1
,
김 하나1
,
김 정동2
,
박 광훈3
,
어 현지4
,
안 미연1
,
정 진부5*
1Graduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Undergraduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
3Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
4Post-doc, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
5Professor, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
1국립안동대학교 생약자원학과, 대학원생
2국립안동대학교 생약자원학과, 학부생
3국립산림과학원 산림약용자원연구소, 연구사
4국립산림과학원 산림약용자원연구소, 박사후연구원
5국립안동대학교 생약자원학과, 교수
* Corresponding Author
30 April 2019. pp. 109-115
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Abstract

In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of branches from Taxillus yadoriki parasitic to Neolitsea sericea (TN-NS-B) against human lung cancer cells, A549. TY-NS-B dose-dependently suppressed the growth of A549 cells. TY-NS-B decreased β-catenin protein level, but not mRNA level in A549 cells. The downregulation of β-catenin protein level by TY-NS-B was attenuated in the presence of MG132. Although TY-NS-B phosphorylated β-catenin protein, the inhibition of GSK3β by LiCl did not blocked the reduction of β-catenin by TY-NS-B. In addition, TY-NS-B decreased β-catenin protein in A549 cells transfected with Flag-tagged wild type β-catenin or Flag-tagged S33/S37/T41 mutant β-catenin construct. Our results suggested that TN-NS-B may downregulate β-catenin protein level independent on GSK3β-induced β-catenin phosphorylation. Based on these findings, TY-NS-B may be a potential candidate for the development of chemopreventive or therapeutic agents for human lung cancer.

Keywords
Anticancer activity
β-catenin
Cancer chemoprevention
Cell growth
Taxillus yadoriki
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :2
  • Pages :109-115
  • Received Date : 2019-01-21
  • Revised Date : 2019-02-28
  • Accepted Date : 2019-03-04
  • DOI :https://doi.org/10.7732/kjpr.2019.32.2.109
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