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2018 Vol.31, Issue 3 Preview Page

Research Article

Ameliorative Effects of Combinative Injection of Ginko biloba Leaves Extract and Vitamin C on Ischemia/Reperfusion Liver Damages Model
Guang-Hua Xie1*
,
Sun Eun Choi2
,
Myung-Jae Mun34
,
Jae-Hun Jeong5
,
Kwang-Hyun Park67
1Department of General Surgery, YanBian University Hospital, JiLin 133-000, China
2Department of Cosmetology Science, Nambu University, Gwangju 62271, Korea
3Department of Biochemical & Polymer Engineering, Chosun University, Gwangju 61452, Korea
4Nano Bio Research Center, JBF, Jangseong 57248, Korea
5Department of Food Science & Bio-Technology, Jeonnam State University, Damyang 57337, Korea
6Department of Oriental Pharmaceutical and Development, Nambu University, Gwangju 62271, Korea
7Department of Emergency Medical Rescue, Nambu University, Gwangju 62271, Korea
* Corresponding Author
30 June 2018. pp. 268-273
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Abstract

Hepatic ischemia-reperfusion injury (HIRI) is linked with high mortality rate. Several agents have been developed so far to reduce the risk of HIRI. In this study, we investigated the effects of combined treatment of Ginko biloba leaves extract and vitamin C (GLEVC) on hepatic ischemia-reperfusion injury. To explore the protective effects of GLEVC on HIRI rats model were tested. After the development of HIRI by using clamping method rats were then randomly divided into four groups. Different doses of GLEVC were administered in HIRI rat model. The level of ALT, AST, SOD and MDA content in serum were detected in HIRI groups. Moreover, the activity of SOD, content of MDA, and GSH in hepatic tissue were also examined. Bcl-2 and Bax protein expression were detected by immunohistochemical staining method. Compared with sham group, GLEVC has the protective effect on the HIRI-induced model. Level of ALT, AST, and MDA in blood were significantly lower in GLEVC group compared with HIRI-induced group. Moreover, SOD activity and GSH were increased in GLEVC group whereas MDA content was reduced by GLEVC treatment. Furthermore, HIRI-induced Bax protein was reduced upon GLEVC treatment, whereas Bcl-2 protein expression was enhanced. These results demonstrate that GLEVC treatment may provide potential ameliorative therapy by reducing damaged signaling mechanism in hepatic ischemia/reperfusion injury model.

Keywords
Apoptosis
Combinative injection
Ginko biloba leaves extract
Hepatic ischemia-reperfusion injury
Vitamin C
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :3
  • Pages :268-273
  • Received Date : 2018-05-24
  • Revised Date : 2018-06-24
  • Accepted Date : 2018-06-25
  • DOI :https://doi.org/10.7732/kjpr.2018.31.3.268
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