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2018 Vol.31, Issue 5 Preview Page

Research Article

Anti-inflammatory Activity of Extracts of Hovenia dulcis on Lipopolysaccharides-stimulated RAW264.7 Cells

LPS로 유도된 RAW264.7 대식세포에 대한 헛개나무(Hovenia dulcis) 추출물의 항염증 효과

Hyun Sim Woo1*
,
Sun Min Lee2*
,
Jeong Doo Heo2
,
Min-Sung Lee3
,
Yeong-Su Kim3
,
Dae Wook Kim3**
우 현심1*
,
이 선민2*
,
허 정두2
,
이 민성3
,
김 영수3
,
김 대욱3**
1Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University
2Gyeongnam Department of Environmental Toxicology and Chemistry, Toxicology Screening Research Center, Korea Institute of Toxicology
3Plant Resource Industry Division, Forest Plant Industry Department, Baekdudaegan National Arboretum
1국립경상대학교 응용생명과학부
2안전성평가연구소
3국립백두대간수목원
* First Author
** Corresponding Author
31 October 2018. pp. 466-477
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Abstract

In this study, the anti-inflammatory activities of the extracts of different parts of Hovenia dulcis such as leaves, stems, and roots were investigated. Among them, the roots extract (RE) showed the most potent suppressive effect against pro-inflammatory mediators in LPS-stimulated mouse macrophage cells. RE induced dose-dependent reduction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and concomitantly reduced the production of NO and PGE2. Additionally, pre-treatment with RE significantly suppressed the production of inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6, as well as mRNA levels. Moreover, phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear translocation of nuclear factor-kappa B (NF-kB) were also strongly attenuated by RE in RAW264.7 cell. Furthermore, RE induced HO-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) and increase HO-1 activity in RAW264.7 macrophages. Therefore, these results indicate that RE strongly inhibits LPS-induced inflammatory responses by blocking NF-kB activation, inhibiting MAPKs phosphorylation, and enhancing HO-1 expression in macrophages, suggesting that RE of H. dulicis and a major component, 27-O-protocatechuoylbetulinic acid could be applied as a valuable natural anti-inflammatory material.

Keywords
Anti-inflammatory effect
Heme oxygenase-1
Hovenia dulcis Thunb.
Pro-inflammatory cytokines
27-O-Protocatechuoylbetulinic acid
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :5
  • Pages :466-477
  • Received Date : 2018-05-29
  • Revised Date : 2018-08-07
  • Accepted Date : 2018-09-18
  • DOI :https://doi.org/10.7732/kjpr.2018.31.5.466
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