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2020 Vol.33, Issue 6 Preview Page

Research Article

Anti-skin Aging Potential of Alcoholic Extract of Phragmites communis Rhizome
Chang Woo Ha1†
,
Sung Hyeok Kim1†
,
Sung Ryul Lee2†
,
Sohee Jang3
,
Seung Namkoong4
,
Sungsil Hong4
,
Hyosun Lim5
,
Youn Kyu Kim6
,
Eun-Hwa Sohn47†
1Graduate Student, College of Health Sciences, Kangwon National University, Samcheok 25949, Korea
2Professor, Department of Convergence Biomedical Science, Cardiovascular and Metabolic Disease Center, College of Medicine, Inje University, Busan 47392, Korea
3Undergraduate Student, College of Health Sciences, Kangwon National University, Samcheok 25949, Korea
4Professor, College of Health Sciences, Kangwon National University, Samcheok 25949, Korea
5Master’s Degree Researcher, Korea Research Institute Bio Science Co., Ltd., Yeoju 12668, Korea
6Post-doc, Korea Research Institute Bio Science Co., Ltd., Yeoju 12668, Korea
7Professor, Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Korea
*ehson@kangwon.ac.kr
†These authors contributed equally to this work.
1 December 2020. pp. 604-614
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Abstract

Chronological aging and photoaging affect appearance, causing wrinkles, pigmentation, texture changes, and loss of elasticity in the skin. Phragmites communis is a tall perennial herb used for its high nutritional value and for medicinal purposes, such as relief from fever and vomiting and facilitation of diuresis. In this study, we investigated the effects of ethanol extract of P. communis rhizome (PCE) on skin aging. The total flavonoid and total phenolic content in PCE were 2.92 ± 0.007 ㎍ of quercetin equivalents (QE) and 231.8 ± 0.001 ㎍ of gallic acid equivalents (GAE) per 100 ㎎ of dried extract (n = 3). The half-maximal inhibitory concentration (IC50) values of PCE for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and hydrogen peroxide scavenging activities were 0.96 and 0.97 ㎎/mL, respectively. PCE showed inhibitory effects on tyrosinase when L-tyrosine (IC50 = 1.25 ㎎/mL) and L-3,4-dihydroxyphenylalanine (IC50 = 0.92 ㎎/mL) were used as substrates. PCE treatment up to 200 ㎍/mL for 24 h did not cause any significant cytotoxicity in B16F10 melanocytes, human dermal fibroblasts (HDFs), and HaCaT keratinocytes. In B16F10 melanocytes, PCE (25 and 50 ㎍/mL) inhibited melanin production and cellular tyrosinase activity after challenge with α-melanocyte-stimulating hormone (α-MSH; p < 0.05). In HDFs, PCE suppressed the mRNA expression of matrix metalloproteinase-1 (MMP-1) and reduced the activity of elastase (p < 0.05). In addition, ultraviolet B (UVB)-mediated downregulation of hyaluronic acid synthase-2 gene expression in HaCaT keratinocytes was also effectively suppressed by PCE treatment. Overall, our results showed that PCE has potential anti-skin aging activity associated with the suppression of hyperpigmentation, wrinkle formation, and reduction in dryness. PCE is a promising candidate for the development of an anti-skin aging cosmetic ingredient.

Keywords
Anti-aging
Elastase
Hyaluronic acid synthase-2
Matrix metalloproteinase-1
Phragmites communis
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :6
  • Pages :604-614
  • Received Date : 2020-06-24
  • Revised Date : 2020-07-30
  • Accepted Date : 2020-08-03
  • DOI :https://doi.org/10.7732/kjpr.2020.33.6.604
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