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2019 Vol.32, Issue 6

Research Article

Combination of Grapefruit and Rosemary Extracts Has Skin Protective Effect through MMPs, MAPKs, and the NF-κB Signaling Pathway In Vitro and In Vivo UVB-exposed Model
Yeo-Cho Yoon1
,
Hee-Jeong Choi4
,
Ji-Hyun Park4
,
Nurud iniyah4
,
Hyun-A Shin2
,
Mi-Yeon Kim3*
1Senior Research Engineer, Natural F&P, Yongin 16827, Korea
2Research Engineer, Natural F&P, Yongin 16827, Korea
3Director, Natural F&P, Yongin 16827, Korea
3Graduate Student, School of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea
* Corresponding Author
31 December 2019. pp. 633-643
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Abstract

Long-term ultraviolet (UV) exposure accelerates the phenomenon of skin photo-aging by activating collagenase and elastase. In this study, we aimed to investigate the effects of a combination of grapefruit and rosemary extracts (cG&Re) on UVB-irradiated damage in HaCaT cells and dorsal mouse skin. In HaCaT cells, cG&Re recovered UVB-reduced cell viability and inhibited protein expression of mitogen-activated protein kinases (MAPKs), such as extracellular signal-regulated kinases (p-Erk), c-Jun N-terminal kinases (p-JNK), and a class of MAPKs (p-P38). Also, cG&Re suppressed UVB-induced collagen and elastin degradation by decreasing matrix metalloproteinases (MMPs) and nuclear factor kappa light chain enhancer of activated B cells (NF-κB) expression, which is a transcription factor. Similar results were observed in dorsal mouse skin. Taken together, our data indicate that cG&Re prevent UVB-induced skin photo-aging due to collagen/elastin degradation via activation of MAPKs, MMPs, and the NF-κB signaling pathway in vitro and in vivo.

Keywords
A combination of grapefruit and rosemary extracts (cG&Re)
Matrix metalloproteinases
Mitogen- activated protein kinases
Skin photo-aging
References
1
Afaq, F. and H. Mukhtar. 2006. Botanical antioxidants in the prevention of photocarcinogenesis and photoaging. Exp. Dermatol. 15(9):678-684.
10.1111/j.1600-0625.2006.00466.x16881964
2
Baek, B., S.H. Lee, K. Kim, H.W. Lim and C.J. Lim. 2016. Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts. Korean J. Physiol. Pharmacol. 20:269-277.
10.4196/kjpp.2016.20.3.26927162481PMC4860369
3
Black, H.S. 2004. Reassessment of a free radical theory of cancer with emphasis on ultraviolet carcinogenesis. Integr. Cancer Ther. 3(4):279-293.
10.1177/153473540427061215523099
4
Bond, M., A.H. Baker and A.C. Newby. 1999. Nuclear factor kappa B activity is essential for matrix metalloproteinase-1 and -3 upregulation in rabbit dermal fibroblasts. Biochem. Biophys. Res. Commun. 264:561-7.
10.1006/bbrc.1999.155110529402
5
Brenneisen, P., H. Sies and K. Scharffetter-Kochanek. 2002. Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann. N. Y. Acad. Sci.973:31-43.
10.1111/j.1749-6632.2002.tb04602.x12485830
6
Chanda, S. and K. Rakholiya. 2011. Combination therapy: Synergism between natural plant extracts and antibiotics against infectious diseases. Microbiol. Book Series 520-529.
7
Choi, H.J., M.B. Alam, P. Zhao, Y.H. Cha, T.H. Kim and S.H. Lee. 2019. Inhibitory Effects of a Combination of Grapefruit and Rosemary Extracts　for Alleviating UV-Induced Skin Ageing. Korean J. Plant Res. 32(3):207-219.
8
Chung, J.H., V.N. Hanft and S, Kang. 2003. Aging and photoaging. J. Am. Acad. Dermatol. 49:690-7.
10.1067/S0190-9622(03)02127-3
9
Cuvelier, M.E., C. Berset and H. Richard. 1994. Antioxidant Constituents in Sage (Salvia officinalis). J. Agric. Food Chem. 42:665-669.
10.1021/jf00039a012
10
Davies, K.J. 2014. The complex interaction of matrix metalloproteinases in the migration of cancer cells through breast tissue stroma. Int. J. Breast Cancer. 839094.
10.1155/2014/83909424800085PMC3985306
11
Doyle, G.A., R.A. Pierce and W.C. Parks. 1997. Transcriptional induction of collagenase-1 in differentiated monocyte-like (U937) cells is regulated by AP-1 and an upstream C/EBP- β site. J. Biol. Chem. 272(18):11840-9.
10.1074/jbc.272.18.118409115242
12
Ficher, G.J, S.C. Datta, H.S. Talwar, Z.Q. Wang, J. Varani, S. Kang and J.J. Voorhees. 1996. Molecular basis of sun- induced premature skin ageing and retinoid antagonism. Nature 379(6563):335-339.
10.1038/379335a08552187
13
Fujii, T., T. Okuda, N. Yasui, M. Wakaizumi, T. Ikami and K. Ikeda. 2013. Effects of amla extract and collagen peptide on UVB-induced photoaging in hairless mice. J. Funct. Foods 5(1):451-459.
10.1016/j.jff.2012.11.018
14
González-Vallinas, M., G. Reglero and A. Ramírez de Molina. 2015. Rosemary (Rosmarinus officinalis L.) Extract as a Potential Complementary Agent in Anticancer Therapy. Nutr. Cancer 67(8):1221-1229.
10.1080/01635581.2015.108211026452641
15
Grace, M.H., Y. Qiang, S. Sang and M.A. Lila. 2017. One- step isolation of carnosic acid and carnosol from rosemary by centrifugal partition chromatography. J. Sep. Sci. 40(5):1057-1062.
10.1002/jssc.20160106328008719
16
Ham, Y.J., V.V. Ortega, F.J. Gomez, O.B. Garcia, J.C. Sanchez and E.S. Min. 2015. Protective effects of rosemary and citrus mixture extracts against photocarcinogenesis in SKH-1 mice model. J. Invest. Cosmetol. 11(4):357-363.
10.15810/jic.2015.11.4.010
17
Jin, G.H., Y. Liu, S.Z. Jin, X.D. Liu and S.Z. Liu. 2007. UVB induced oxidative stress in human keratinocytes and protective effect of antioxidant agents. Radiat. Environ. Biophys. 46(1):61-68.
10.1007/s00411-007-0096-117279358
18
Jun, Y.K., M.H. Kim, S.P. Nam and M.J. Chang. 2012. A comparison of anti-inflammatory activities of green tea and grapefruit seed extract with those of microencapsulated extracts. Korean J. Nutr. 45(5):443-451 (in Korean).
10.4163/kjn.2012.45.5.443
19
Karin, M. 1995. The regulation of AP-1 activity by mitogen- activated protein kinases. J. Biol. Chem. 270(28): 16483-6.
10.1074/jbc.270.28.164837622446
20
Khan, I.A. and E.A. Abourashed. 2011. Leung>s encyclopedia of common natural ingredients: used in food, drugs and cosmetics, John Wiley & Sons, NJ (USA). pp. 1-845.
21
Kim, M., Y.G. Park, H. Lee, S.J. Lim and C.W. Nho. 2015. Youngiasides A and C Isolated from Youngia denticulatum inhibit UVB‐induced MMP expression and promote type I procollagen production via repression of MAPK/AP‐1/NF‐κB and activation of AMPK/Nrf 2 in HaCaT cells and human dermal fibroblasts. J. Agric. Food Chem. 63(22):5428-38.
10.1021/acs.jafc.5b0046725994852
22
Moore, J., M. Yousef and E. Tsiani. 2016. Anticancer effects of rosemary (Rosmarinus officinalis L.) extract and rosemary extract polyphenols. Nutrients 8(11):E731.
10.3390/nu811073127869665PMC5133115
23
Naylor, E.C., R.E. Watson and M.J. Sherratt. 2011. Molecular aspects of skin ageing. Maturitas 69(3):249-256.
10.1016/j.maturitas.2011.04.01121612880
24
Park, J.E., H.B. Pyun, S.W. Woo, J.H. Jeong and J.K. Hwang. 2014. The protective effect of Kaempferia parviflora extract on UVB-induced skin photoaging in hairless mice. Photodermatol Photoimmunol Photomed 30(5):237-245.
10.1111/phpp.1209724313661
25
Park, H.K. and S.B Kim. 2006. Antimicrobial activity of grapefruit seed extract. Korean J. Food & Nutr. 19(4):526- 531 (in Korean).
26
Petiwala, S.M., A.G. Puthenveetil and J.J. Johnson. 2013. Polyphenols from the Mediterranean herb rosemary (Rosmarinus officinalis) for prostate cancer. Front. Pharmacol. 4:29.
10.3389/fphar.2013.0002923531917PMC3607075
27
Pittayapruek, P., J. Meephansan, O. Prapapan, M. Komine and M. Ohtsuki. 2016. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int. J. Mol. Sci. 17(6): E868.
10.3390/ijms1706086827271600PMC4926402
28
Quan, T., Z. Qin, W. Xia, Y. Shao, J.J. Voorhees and G.J. Fisher. 2009. Matrix-degrading metalloproteinases in photoaging. J. Investig. Dermatol. Symp. Proc. 14(1):20-4.
10.1038/jidsymp.2009.819675548PMC2909639
29
Rabe, J.H., A.J. Mamelak, P.J. McElgunn, W.L. Morison and D.N. Sauder. 2006. Photoaging: mechanisms and repair. J. Am. Acad. Dermatol. 55(1):1-19.
10.1016/j.jaad.2005.05.01016781287
30
Rittie, L. and G.J. Fisher. 2002. UV-light-induced signal cascades and skin aging. Ageing Res. Rev. 1(4):705-20.
10.1016/S1568-1637(02)00024-7
31
Rosenthal, F.S., A.E. Bakalian and H.R. Taylor. 1986. The effect of prescription eyewear on ocular exposure to ultraviolet radiation. Am. j. public health 76(10): 1216-1220.
10.2105/AJPH.76.10.12163752323PMC1646665
32
Sun, Z.W., E. Hwang, H.J. Lee, T.Y. Lee, H.G. Song, S.Y. Park, H.S. Shin, D.G. Lee and T.H. Yi. 2015. Effects of Galla chinensis extracts on UVB-irradiated MMP-1 production in hairless mice. J. Nat. Med. 69(1):22-34.
10.1007/s11418-014-0856-625227288
33
Vanamala, J., L. Reddivari, K.S. Yoo, L.M. Pike and B.S. Patil. 2006. Variation in the content of bioactive flavonoids in different brands of orange and grapefruit juices. J. Food Compost. Anal. 19(2-3):157-166.
10.1016/j.jfca.2005.06.002
34
Wolfle, U., P.R. Esser, B. Simon-Haarhaus, S.F. Martin, J. Lademann and C.M. Schempp. 2011. UVB-induced DNA damage, generation of reactive oxygen species, and inflammation are effectively attenuated by the flavonoid luteolin in vitro and in vivo. Free Radic. Biol. Med. 50(9):1081-1093.
10.1016/j.freeradbiomed.2011.01.02721281711
35
Xiaojuan, L., D. Jiquan, O. Yangwen, X. Hong, C. Xuexiang, Z. Aimei, H. Liping and C. Yong. 2013. Degradation pathway of carnosic acid in methanol solution through isolation and structural identification of its degradation products. Eur. Food Res. Technol. 237(4):617-626.
10.1007/s00217-013-2035-5
36
Yoshizumi, M, T. Nakamura, M. Kato, T. Ishioka, K. Kozawa, K. Wakamatsu and H. Kimura. 2008. Release of cytokines/ chemokines and cell death in UVB-irradiated human keratinocytes, HaCaT. Cell Biol. 32(11):1405-11.
10.1016/j.cellbi.2008.08.01118782623
37
Zhang, Y., J.P. Smuts, E. Dodbiba, R. Rangarajan, J.C. Lang and D.W. Armstrong. 2012. Degradation study of carnosic acid, carnosol, rosmarinic acid, and rosemary extract (Rosmarinus officinalis L.) assessed using HPLC. J. Agric. Food Chem. 60(36):9305-9314.
10.1021/jf302179c22881034
38
Zhou, X., S.W. Seto, D. Chang, H. Kiat, V. Razmovski- Naumovski, K. Chan and A. Bensoussan. 2016. Synergistic effects of Chinese herbal medicine: a comprehensive review of methodology and current research. Front. Pharmacol. 7:201.
10.3389/fphar.2016.00201
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :6
  • Pages :633-643
  • Received Date : 2019-09-10
  • Revised Date : 2019-10-08
  • Accepted Date : 2019-10-30
  • DOI :https://doi.org/10.7732/kjpr.2019.32.6.633
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