All Issue

2018 Vol.31, Issue 5 Preview Page

Research Article

Anti-inflammatory Effects of Various Mushrooms in LPS-stimulated RAW264.7 Cells
Kyung Hye Seo1*
,
Jeong-Yong Park23
,
Hyung-Jun Noh1
,
Ji Yeon Lee23
,
Eun Young Lee2
,
Jae-Gu Han4
,
Jin Hyo Kim5
,
Mi Sun Cheong6
1Researcher, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science
2Assistant, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science
3Student, Department of Food Science and Biotechnology, Chungbuk National University
4Researcher, Mushroom Research Division, National Institute of Horticultural & Herbal Science
5Professor, Institute of Agriculture and Life Science (IALS), Gyeongsang National University
6Researcher, Institute of Agriculture and Life Science (IALS), Gyeongsang National University
* Corresponding Author
31 October 2018. pp. 478-488
PDF XML Citation
Abstract

Mushrooms have been widely cultivated and consumed as foods and herbal medicines owing to their various biological properties. However, few studies have evaluated the anti-inflammatory effects of mushrooms. Here, we investigated the effects of mushroom extracts (MEs) on lipopolysaccharide (LPS)-induced inflammation in macrophages (RAW264.7 cells). First, we extracted MEs with either water or ethanol. Using LPS-treated RAW264.7 cells, we measured cell proliferation and NO production. Gene expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-6 (IL-6), and IL-1β was assessed by RT-PCR, and protein abundance of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) and phosphorylation of p65 were determined by immunoblotting. MEs prepared using both water and ethanol inhibited LPS-induced inflammation in RAW264.7 cells. Nitric oxide (NO) levels induced by LPS were reduced by treatment with MEs. Isaria japonica Yasuda water extracts and Umbilicaria esculenta (Miyoshi) Minks ethanol extracts significantly decreased the mRNA expression of inflammation-related cytokine genes including TNF-α, IL-6, and IL-1β. Similarly, the protein abundance of iNOS and COX-2 was also decreased. The phosphorylation of p65, a subunit of nuclear factor-κB was at least partly suppressed by MEs. This study suggests that mushrooms could be included in the diet to prevent and treat macrophage-related chronic immune diseases.

Keywords
Anti-inflammation
Isaria japonica Yasuda
Macrophage
Medicinal mushrooms
Umbilicaria esculenta (Miyoshi) Minks
References
1
Aktan, F. 2004. iNOS-mediated nitric oxide production and its regulation. Life Sci. 75:639-653.
10.1016/j.lfs.2003.10.04215172174
2
Alvarez, S. and P.A. Evelson. 2007. Nitric oxide and oxygen metabolism in inflammatory conditions: sepsis and exposition to polluted ambients. Front Biosci. 12:964-974.
10.2741/211717127352
3
Chien, R.C., Y.C. Yang, E.I. Lai and J.L. Mau. 2016. Anti- inflammatory effects of extracts from the medicinal mushrooms Hypsizygus marmoreus and Pleurotus eryngii (Agaricomycetes). International Journal of Medicinal Mushrooms 18:477-487.
10.1615/IntJMedMushrooms.v18.i6.20
4
Du, C., M. Bhatia, S.C. Tang, M. Zhang and T. Steiner. 2015. Mediators of inflammation: Inflammation in cancer, chronic diseases, and wound healing. Mediators Inflamm. 2015:570653.
10.1155/2015/57065326549940PMC4624916
5
Gunawardena, D., L. Bennett, K. Shanmugam, K. King, R. Williams, D. Zabaras, R. Head, L. Ooi, E. Gyengesi and G. Münch. 2014. Anti-inflammatory effects of five commercially available mushroom species determined in lipopolysaccharide and interferon-γ activated murine macrophages. Food Chemistry 148:92-96.
10.1016/j.foodchem.2013.10.01524262531
6
Guzik, T.J., R. Korbut and T. Adamek-Guzik. 2003. Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Pharmacol. 54:469-487.PMid:14726604
7
Kim, H.H., S.Y. Lee, T.S. Singh, J.K. Choi, T.Y. Shin and S.H. Kim. 2012. Sparassis crispa suppresses mast cell-mediated allergic inflammation: Role of calcium, mitogen-activated protein kinase and nuclear factor-κB. International Journal of Molecular Medicine 30:344-350.
10.3892/ijmm.2012.100022614038
8
Kuo, M.C., C.Y. Weng, C.L. Ha and M.J. Wu. 2006. Ganoderma lucidum mycelia enhance innate immunity by activating NF-κB. Journal of Ethnopharmacology 103:217-222.
10.1016/j.jep.2005.08.01016169168
9
Libby, P., P.M. Ridker and A. Maseri. 2002. Inflammation and atherosclerosis. Circulation 105:1135-1143.
10.1161/hc0902.10435311877368
10
Lin, J.T., C.W. Liu, Y.C. Chen, C.C. Hu, L.D. Juang, C.C. Shiesh and D.J. Yang. 2014. Chemical composition, antioxidant and anti-inflammatory properties for ethanolic extracts from Pleurotus eryngii fruiting bodies harvested at different time. LWT-Food Science and Technology 55:374-382.
10.1016/j.lwt.2013.08.023
11
Lull, C., H.J. Wichers and H.F. Savelkoul. 2005. Antiinflammatory and immunomodulating properties of fungal metabolites. Mediators Inflamm. 2005:63-80.
10.1155/MI.2005.6316030389PMC1160565
12
Ma, L., H. Chen, P. Dong and X. Lu. 2013. Anti-inflammatory and anticancer activities of extracts and compounds from the mushroom Inonotus obliquus. Food Chemistry 139:503-508.
10.1016/j.foodchem.2013.01.03023561137
13
Mann, J.R., M.G. Backlund and R.N. DuBois. 2005. Mechanisms of disease: Inflammatory mediators and cancer prevention. Nat Clin Pract Oncol. 2:202-210.
10.1038/ncponc014016264935
14
O'Connell, M.A., B.L. Bennett, F. Mercurio, A.M. Manning and N. Mackman. 1998. Role of IKK1 and IKK2 in lipopolysaccharide signaling in human monocytic cells. J Biol Chem. 273:30410- 30414.
10.1074/jbc.273.46.304109804806
15
Pacher, P., J.S. Beckman and L. Liaudet. 2007. Nitric oxide and peroxynitrite in health and disease. Physiol Rev. 87:315-424.
10.1152/physrev.00029.200617237348PMC2248324
16
Park, S.B., H.M. Song, H.N. Kim, G.H. Park, H.J. Son, Y. Um, J.A. Park and J.B. Jeong. 2018. Anti-inflammatory effect of Biji (Soybean curd residue) on LPS-stimulated RAW264.7 Cells. Korean J. Plant Res. 31:117-123.
17
Park, Y.M., J.H. Won, Y.H. Kim, J.W. Choi, H.J. Park and K.T. Lee. 2005. In vivo and in vitro anti-inflammatory and anti- nociceptive effects of the methanol extract of Inonotus obliquus. Journal of Ethnopharmacology 101:120-128.
10.1016/j.jep.2005.04.00315905055
18
Radic, N., Z. Jevnikar, N. Obermajer, J. Kristl, J. Kos, F. Pohleven and B. Strukelj. 2010. Infuence of culinary- medicinal Maitake mushroom, Grifola frondosa (Dicks.: Fr.) SF Gray (Aphyllophoromycetideae) polysaccharides on gene expression in Jurkat T lymphocytes. International Journal of Medicinal Mushrooms 12:245-255.
10.1615/IntJMedMushr.v12.i3.30
19
Reid, T., M. Munyanyi and T. Mduluza. 2017. Effect of cooking and preservation on nutritional and phytochemical composition of the mushroom Amanita zambiana. Food Sci Nutr. 5:538- 544.
10.1002/fsn3.42828572939PMC5448345
20
Robaszkiewicz, A., G. Bartosz, M. Lawrynowicz and M. Soszynski. 2010. The role of polyphenols, beta-carotene, and lycopene in the antioxidative action of the extracts of dried, edible mushrooms. J Nutr Metab. 2010:173274.
10.1155/2010/17327421234347PMC3017951
21
Trianthi, P. and A. Aggarwal. 2006. NF-kB transcription factor: a key player in the generation of immune response. Current Science 90:519-531.
22
Taofiq, O., R.C. Calhelha, S. Heleno, L. Barros, A. Martins, C. Santos-Buelga, M.J.R. Queiroz and I.C. Ferreira. 2015. The contribution of phenolic acids to the anti-inflammatory activity of mushrooms: Screening in phenolic extracts, individual parent molecules and synthesized glucuronated and methylated derivatives. Food Research International. 76:821-827.
10.1016/j.foodres.2015.07.04428455068
23
Tsai, P.J., T.H. Tsai, C.H. Yu and S.C. Ho. 2007. Evaluation of NO-suppressing activity of several Mediterranean culinary spices. Food Chem Toxicol. 45:440-447.
10.1016/j.fct.2006.09.00617074427
24
Van, Q., B. Nayak, M. Reimer, P. Jones, R. Fulcher and C. Rempel. 2009. Anti-inflammatory effect of Inonotus obliquus, Polygala senega L., and Viburnum trilobum in a cell screening assay. Journal of Ethnopharmacology 125:487-493.
10.1016/j.jep.2009.06.02619577624
25
Wasser, S.P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol. 60:258-274.
10.1007/s00253-002-1076-712436306
26
Wink, D.A. and J.B. Mitchell. 1998. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med. 25:434-456.
10.1016/S0891-5849(98)00092-6
27
Yi, M., C. Kang and H. Bu. 2017. Anti-inflammatory and tyrosinase inhibition effects of amaranth (Amaranthus spp. L.) seed extract. Korean J. Plant Res. 30:144-151.
10.7732/kjpr.2017.30.2.144
28
Yoon, H.M., K.J. Jang, M.S. Han, J.W. Jeong, G.Y. Kim, J.H. Lee and Y.H. Choi. 2013. Ganoderma lucidum ethanol extract inhibits the inflammatory response by suppressing the NF-κB and toll-like receptor pathways in lipopolysaccharide- stimulated BV2 microglial cells. Experimental and Therapeutic Medicine 5:957-963.
10.3892/etm.2013.89523408713PMC3570243
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :5
  • Pages :478-488
  • Received Date : 2018-05-18
  • Revised Date : 2018-07-24
  • Accepted Date : 2018-07-24
  • DOI :https://doi.org/10.7732/kjpr.2018.31.5.478
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close